元宝枫种仁多糖提取纯化、结构表征以及降糖活性研究
Study on Extraction,Purification,Structure Characterization and Hypoglycemic Activity of the Seed Kernel Polysaccharide from Acer truncatum Bunge
米圣成 1徐晓杰 2魏菱鸽 1路祺 2朱明华 3包怡红 1陈春霞2
作者信息
- 1. 东北林业大学生命科学学院,黑龙江哈尔滨 150000
- 2. 东北林业大学化学与资源利用学院,黑龙江哈尔滨 150000
- 3. 哈尔滨职业技术学院,黑龙江哈尔滨 150000
- 折叠
摘要
对元宝枫(Acer truncatum Bunge)种仁多糖进行提取纯化,探究其理化性质和降糖活性.采用水提醇沉法提取元宝枫种仁多糖,并通过响应面法优化元宝枫种仁多糖提取工艺.通过Sevage法脱蛋白和柱层析对元宝枫种仁多糖进行纯化,通过红外光谱、热重分析及离子色谱法对其进行单糖组成和结构表征,并进一步通过建立肝癌细胞胰岛素抵抗模型(IR-HepG2),考察纯化后元宝枫种仁多糖体外降糖活性.结果表明,在提取 1次、液固比为 30:1 mL/g、提取温度为 80℃、提取时间为 3.5 h条件下,多糖(PATM)得率为 18.17%.经Sevage法脱蛋白,DEAE-DE纤维素52层析柱和SephadexG-100凝胶层析柱分离纯化,得到纯化多糖(PATM-3-1).PATM-3-1具有多糖组分红外光谱特征峰,其单糖组成为D-半乳糖醛酸、L-阿拉伯糖、D-半乳糖、L-鼠李糖、D-葡萄糖、D-木糖、D-甘露糖、D-葡萄糖醛酸、L-岩藻糖、D-核糖和D-氨基葡萄糖.体外降糖活性实验的结果表明,PATM-3-1具有较强的α-淀粉酶抑制活性,能显著(P＜0.05)提高胰岛素诱导的HepG2细胞中葡萄糖消耗量、糖原含量、己糖激酶、丙酮酸激酶含量,展现出优异的降糖活性.本研究为元宝枫种仁多糖的开发和应用提供参考和数据支撑.
Abstract
Extraction and purification of polysaccharides from the seed kernel of Acer truncatum Bungeto investigate their physicochemical properties and hypoglycemic activity.In the study,the polysaccharide was obtained by water extraction and ethanol precipitation from seed kernel of Acer truncatum Bunge,and the extraction process was optimized by response surface method.The polysaccharide was purified by Sevage deproteinization and column chromatography.The composition and structure of the purified polysaccharide were characterized by infrared spectroscopy,thermogravimetric analysis and ion chromatography,and its hypoglycemic activity was further investigated by the insulin resistance model of liver cancer cell(IR-HepG2).The results showed that the yield of crude polysaccharide(PATM)was 18.17%under the conditions of extraction times 1,liquid-solid ratio 30:1 mL/g,extraction temperature 80℃and extraction time 3.5 h.The purified polysaccharide(PATM-3-1)obtained by the Sevage deproteinization,DEAE-DE cellulose 52 column and SephadexG-100 gel column had the characteristic peak of infrared spectrum of polysaccharide component,and was composed of D-galacturonic acid,L-arabinose,D-galactose,L-rhamnose,D-glucose,D-xylose,D-mannose,D-glucuronic acid,L-fucose,D-ribose and D-glucosamine.The results of hypoglycemic activity experiment in vitro showed that PATM-3-1 had strong α-amylase inhibitory activity,and could significantly(P＜0.05)increase the glucose consumption,glycogen content,hexokinase content and pyruvate kinase content in IR-HepG2,demonstrating excellent hypoglycemic activity.This study would provide data support for the development and application of seed kernel polysaccharide from Acer truncatum Bunge.
关键词
元宝枫种仁多糖/响应面/分离纯化/单糖组成/降糖活性/HepG2细胞Key words
seed kernel polysaccharide of Acer truncatum Bunge/response surface/separation and purification/monosaccharide composition/hypoglycemic activity/HepG2 cells引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据