HPLC-FP法同时测定新品富硒竹笋中10种核苷类成分的含量
Simultaneous Determination of Ten Nucleosides in New Selenium-enriched Bamboo Shoots by HPLC-FP
秦心睿 1聂晓兵 1袁高阳 1刘诗菡 1杨玉玉 1刘志新 2邱首哲 2郑丽 2杨硕 2范宝磊3
作者信息
- 1. 湖北科技学院药学院,湖北咸宁 437100
- 2. 湖北省咸宁市农业科学院,湖北咸宁 437100
- 3. 湖北科技学院核技术与化学生物学院,湖北咸宁 437100
- 折叠
摘要
目的:建立同时测定不同品种富硒竹笋中胞嘧啶、尿嘧啶、胞苷、次黄嘌呤、尿苷、胸腺嘧啶、腺嘌呤、鸟苷、胸苷、腺苷 10种核苷类化合物含量的方法.方法:运用HPLC法同时测定 21种不同品种竹笋的核苷类化合物含量,色谱柱为 Inertsil ODS-3 C18,流动相为乙腈-水(梯度洗脱),检测波长为 260 nm,流速为0.8 mL·min-1,柱温为 30℃,进样量为 20 μL.绘制 21种富硒竹笋的指纹图谱进行相似度评价,确定共有峰,并用主成分分析、相关性分析和聚类分析对 21种不同富硒竹笋的核苷类化合物含量测定结果进行分析.结果:10种核苷类成分在 30 min内达到分离,线性关系良好(R2≥0.9990),精密度、稳定性、重复性、准确度试验RSD均小于 2%.不同品种富硒竹笋中 10种核苷类成分含量与结构比差异明显,并且核苷类化合物成分指标互相间存在着不同程度的相关性.通过主成分分析,提取了 4个主成分,并确定了胸苷、胸腺嘧啶、腺苷、鸟苷为 4项核心指标,可用于评价不同品种富硒竹笋的品质;通过相关性分析,得出尿嘧啶与胞苷、鸟苷与胸苷、胸苷与腺苷等指标间相关性较高,指标之间的含量会互相影响;通过聚类分析,得出新品富硒竹笋主要集中为一类,市场上常见的富硒竹笋主要分为两类.结论:该实验方法操作快速、简便,重现性好,可用于 21种富硒竹笋中 10种核苷类化合物的含量测定.同时,测定结果为竹笋的后期高效开发和利用提供理论依据.
Abstract
Objective:To establish a method for simultaneous determination of cytosine,uracil,cytidine,hypoxanthine,uridine,thymidine,adenine,guanosine,thymidine and adenosine in different selenium-rich bamboo shoots.Methods:The determination was performed on Inertsil ODS-3 C18 column with mobile phase consisting of acetonitrile-water(gradient elution).The detection wavelength was set at 260 nm and the flow rate was 0.8 mL·min-1.The column temperature was 30℃,and the sample size was 20 μL.Fingerprints of 21 selenium-rich bamboo shoots were drawn for similarity evaluation of common peaks.The nucleosides of 21 selenium-rich bamboo shoots were analysised by principal component analysis,correlation analysis and cluster analysis.Results:10 nucleosides were separated within 30 min,and the linear relationship was good(R2≥0.9990).RSDS of precision,stability,repeatability and accuracy tests were all less than 2%.Significant differences in the contents and structure ratio of 10 nucleosides were found in different selenium-rich bamboo shoots,and there were different degrees of correlation between nucleoside components.Four principal components were selected by principal component analysis.Thymidine,thymine,adenosine and guanosine were identified as four core indexes,which could be used to evaluate the quality of different selenium-rich bamboo shoots.The correlation between uracil and cytidine,guanosine and thymidine,thymidine and adenosine was high,and the contents of these indexes would influence each other.After cluster analysis,it was concluded that the new selenium-rich bamboo shoots were mainly concentrated in one category,and the common selenium-rich bamboo shoots in the market are mainly divided into two categories.Conclusion:The method was rapid,simple and reproducible,and could be used for the determination of 10 nucleosides in 21 selenium-rich bamboo shoots.At the same time,the results would provide a theoretical basis for the efficient development and utilization of bamboo shoots in the later period.
关键词
核苷类化合物/富硒竹笋/高效液相色谱法(HPLC)/指纹图谱/主成分分析/相关性分析/聚类分析Key words
nucleoside compounds/bamboo shoots/high performance liquid chromatography(HPLC)/finger-print/principal component analysis/correlation analysis/cluster analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据