响应面法优化准噶尔山楂总三萜提取工艺及其纯化工艺研究
Optimization of Extraction Process of Total Triterpenoids from Crataegus songarica by Response Surface Methodology and Its Purification Process
冯琳 1罗世博 1古丽格娜·皮达买买提 2刘媛梦 3高红艳1
作者信息
- 1. 伊犁师范大学化学化工学院,新疆伊宁 835000;新疆普通高等学校天然产物化学与应用重点实验室,新疆伊宁 835000
- 2. 伊犁师范大学化学化工学院,新疆伊宁 835000
- 3. 伊犁师范大学化学化工学院,新疆伊宁 835000;污染物化学与环境治理重点实验室,新疆伊宁 835000
- 折叠
摘要
为了提高准噶尔山楂的高值化利用,以准噶尔山楂为原料,通过单因素实验、正交试验和响应面设计优化复合酶辅助提取准噶尔山楂总三萜的工艺,并对纯化工艺进行优化.结果表明,准噶尔山楂总三萜的最优提取工艺为纤维素酶、果胶酶和木瓜蛋白酶的添加量分别为4％、4％和2％,料液比为1∶24g/mL,温度51 ℃,pH为4.5,酶解76 min,此条件下准噶尔山楂总三萜的提取量为(36.570±0.332)mg/g,通过超声再次提取,总三萜的提取量达到(53.782±0.673)mg/g.纯化工艺为:上样液浓度为6 mg/mL,流速:1 mL/min,上样量:15 mL,洗脱条件为50 mL,70％乙醇,纯化后的准噶尔山楂总三萜的纯度可达29.93％,回收率为81.25％.以上结果说明复合酶法对准噶尔山楂总三萜的提取和采用大孔树脂纯化具有可行性且稳定性高.
Abstract
In order to improve the high-value utilization of Crataegus songarica,the single factor experiment,orthogonal experiment and response surface design were used to optimize the extraction process of total triterpenes from Crataegus songarica by compound enzyme assisted extraction,and the purification process was optimized.The results showed that the optimal extraction process of total triterpenes from Crataegus songarica was as follows:The addition amount of cellulase,pectinase and papain was 4％,4％and 2％,respectively,the ratio of material to liquid was 1∶24 g/mL,the temperature was 51 ℃,the pH was 4.5,and the enzymolysis time was 76 min.Under these conditions,the extraction amount of total triterpenes from Crataegus songarica was(36.570±0.332)mg/g,and the extraction amount of total triterpenes was(53.782±0.673)mg/g by ultrasonic extraction again.The purification process was as follows:The concentration of the sample solution was 6 mg/mL,the flow rate was 1 mL/min,the sample volume was 15 mL,the elution condition was 50 mL,and 70％ethanol.The purity of the total triterpenoids was 29.93％,and the recovery rate was 81.25％.The above results indicate that the compound enzymatic method is feasible and stable for the extraction and purification of total triterpenoids from Crataegus songarica by macroporous resin.
关键词
准噶尔山楂/三萜/酶辅助提取/大孔树脂/纯化Key words
Crataegus songarica/triterpenes/enzyme-assisted extraction/macroporous resin/purification引用本文复制引用
基金项目
新疆普通高等学校天然产物化学与应用重点实验室开放基金(2023YSHXYB01)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据