QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留
Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
刘华文 1邓美晴 2陈北燕3
作者信息
- 1. 广西-东盟食品检验检测中心,广西南宁 530029
- 2. 广西医科大学,公共卫生学院,广西南宁 530022
- 3. 南宁市卫生学校,广西南宁 530409
- 折叠
摘要
建立一种QuEChERS-超高效液相色谱-串联质谱法同时测定茶叶中47种农药残留的分析方法.样品经1％乙酸乙腈提取,QuEChERS方法净化,配有电喷雾离子源(ESI±)的超高效液相色谱-串联质谱(UPLC-MS/MS),在正负离子多反应监测(MRM)模式下同时测定,基质匹配外标法定量.结果表明:47种目标化合物在一定范围内线性关系良好,相关系数(r)为0.9971～0.9996,检出限为0.001～0.006 mg/kg,定量限为0.002～0.020 mg/kg,平均回收率为73.4％～114.3％,相对标准偏差(n=6)为0.3％～19.9％.该方法灵敏、准确、稳定,可满足茶叶中47种农药残留的检测要求.
Abstract
An analysis method for simultaneous determination of 47 kinds of pesticide residues in tea was established by QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry.The samples were extracted by acetonitrile containing 1％acetic acid,and purified by QuEChERS method.The 47 pesticides were determined by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with an electrospray ion source(ESI±)in positive and negative ion switching multiple reaction monitoring(MRM),and quantified by matrix-matched external standard method.Method validation revealed satisfactory linearities with correlation coefficients(r)between 0.9971 and 0.9996,and limits of detection were 0.001～0.006 mg/kg while the limits of quantitation were 0.002～0.020 mg/kg,the average recoveries at three spiked levels were in the range of 73.4％～114.3％,with relative standard deviations(n=6)of 0.3％～19.9％.The method is sensitive,accurate,stable,and can meet the requirements for determination of 47 pesticide residues in tea.
关键词
QuEChERS/茶叶/农药残留/超高效液相色谱-串联质谱法(UPLC-MS/MS)Key words
QuEChERS/tea/pesticide residues/ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据