槟榔碱对人胃黏膜上皮细胞的毒性作用研究
Toxic Effects of Arecoline on Human Stomach Mucosal Epithelial Cells
李家旭 1成钰莹 1杨扬 1王璧莹 1蒋玉珍 1陈建萍 2黎攀 1杜冰1
作者信息
- 1. 华南农业大学食品学院,广东广州 510642
- 2. 香港大学中医药学院,中国香港 999077
- 折叠
摘要
本文旨在探讨槟榔碱对人胃黏膜上皮细胞GES-1的毒性作用.用不同浓度(60、90、120、150μg/mL)的槟榔碱处理GES-1细胞,并检测GES-1细胞活力、活性氧水平(Reactive Oxygen Species,ROS)和线粒体膜电位(Mitochondrial Membrane Potential,MMP),测定 GES-1 细胞还原型谷胱甘肽(Glutathione,GSH)水平、超氧化物歧化酶(Superoxide Dismutase,SOD)活力、乳酸脱氢酶(Lactate dehydrogenase,LDH)活力、三磷酸腺苷(ATP)酶活力.测定肿瘤坏死因子-α(TumorNecrosis Factor-α,TNF-α)、白介素-6(Interleukin 6,IL-6)、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)、转化生长因子-β(Transforming Growth Factor-β,TGF-β)水平.结果表明,与空白对照组相比,槟榔碱刺激后GES-1细胞形态发生改变,细胞活力最低降低至24％,活性氧水平最高升至12.5倍,线粒体膜电位最低下降至10.4％.在150 μg/mL槟榔碱刺激下,总 ATP酶以及Na+K+-ATP酶活力分别下降至42.31％和39.84％,GSH和SOD分别下降了 40.23％和34.1％,LDH上升了30.46％,TNF-α、IL-6、VEGF、TGF-β 水平分别上升了 4.67％、10.2％、23.14％和 22.83％.结果表明槟榔碱能够产生氧化应激并诱发炎症因子,对GES-1细胞造成毒性损伤.
Abstract
The purpose of this study was to look into the toxicity of arecoline on human stomach mucosal epithelial cells GES-1.GES-1 cells were treated with different concentrations(60,90,120,150 μg/mL)of arecoline,detection of GES-1 cell viability,reactive oxygen species levels(ROS)and mitochondrial membrane potential(MMP).The levels of glutathione(GSH),superoxide dismutase(SOD),lactate dehydrogenase(LDH),adenosine triphosphate(ATP)enzymes were measured in GES-1 cellls.And the leves of tumor necrosis factor-α(TNF-α),interleukin 6(IL-6),vascular endothelial growth factor(VEGF),transforming growth factor-β(TGF-β)were measured in GES-1 cells.Compared with the control group,the shape of GES-1 cells was altered after arecoline stimulation,with the lowest cell viability rate reduced to 24％,the highest reactive oxygen species level increased to 12.5 fold,and the lowest mitochondrial membrane potential decreased to 10.4％.Under 150 μg/mL arecoline stimulation,T-ATPase and Na+K+-ATPase activity decreased to 42.31％and 39.84％,GSH and SOD decreased by 40.23％and 34.1％,respectively,LDH increased by 30.46％,TNF-α,IL-6,VEGF and TGF-βlevels increased by 4.67％,10.2％,23.14％and 22.83％.The results indicate that arecoline can produce oxidative stress and induce inflammatory factors that cause toxic damage to GES-1 cells.
关键词
槟榔碱/人胃黏膜上皮细胞GES-1/氧化应激/线粒体/细胞毒性Key words
arecoline/human gastric mucosal epithelial cells GES-1/oxidative stress/mitochondria/cytotoxic引用本文复制引用
基金项目
广东省现代农业产业技术体系建设专项创新团队项目(2023KJ125)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据