亚临界水萃取薄荷黄酮工艺优化及神经保护活性研究
Optimization of Extraction Process and Neuroprotective Activities of Flavonoids from Mentha haplocalyx Briq.with Subcritical Water
张晴晴 1张梦娇 2马翊铭 3蔡学一3
作者信息
- 1. 亳州学院中药学院,安徽亳州 236800;江南大学化学与材料工程学院,江苏无锡 214000
- 2. 安徽雷允上药业有限公司,安徽亳州 236800
- 3. 亳州学院中药学院,安徽亳州 236800
- 折叠
摘要
目的:优化亚临界水萃取薄荷黄酮提取工艺,探究薄荷黄酮对H2O2 诱导的大鼠肾上腺嗜铬细胞瘤(PC12细胞)损伤的缓解作用.方法:采用单因素实验、Box-Behnken响应面试验优化亚临界水萃取薄荷黄酮工艺,并与回流提取法、超声波辅助提取法、超临界CO2 萃取法进行比较.体外培养PC12细胞,建立H2O2 诱导的PC12细胞损伤模型,噻唑蓝(methyl thiazolye tetrazolium,MTT)法考察薄荷黄酮对PC12细胞存活力的影响,分析神经保护活性.结果:最佳提取工艺为提取温度 142℃、提取时间 38 min、液料比 21:1 mL/g,在此条件下得到薄荷黄酮得率为 14.07%±0.23%,比回流提取法提高了 334%,比超声波辅助提取法提高了 75%,比超临界CO2 萃取法提高了 173%,显著高于以上 3种提取方法的得率(P<0.001).与H2O2 组比较,薄荷黄酮在 1～100µg/mL浓度范围内,使PC12细胞存活率从 53.61%±0.64%显著(P<0.001)提高到 78.49%±0.84%,表明薄荷黄酮能明显缓解细胞氧化损伤.结论:建立了一套绿色、高效、可靠的亚临界水萃取薄荷黄酮的工艺,萃取的薄荷黄酮对H2O2 诱导的PC12细胞损伤具有极显著的保护作用(P<0.01).
Abstract
Objective:To optimize the subcritical water extraction process for flavonoids from Mentha haplocalyx Briq.(mint)and assess the mitigating effects of peppermint flavonoids on H2O2-induced adrenal pheochromocytoma(PC12 cell)injury in rats.Methods:The subcritical water extraction process for mint flavonoids was optimized using single-factor and Box-Behnken response surface tests.A comparison was made with reflux extraction,ultrasonic-assisted extraction,and supercritical CO2 extraction methods.In vitro cell culture of PC12 cells was employed to establish an H2O2-induced PC12 cell injury model.The neuroprotective activity of mint flavonoids was analyzed using the MTT(methyl thiazolye tetrazolium,MTT)method.Results:The optimized extraction conditions were determined to be 142℃,38 min,and 21:1 mL/g,resulting in a mint flavonoid yield of 14.07%±0.23%.This yield was significantly higher(P<0.001)than that of reflux extraction(334%increase),ultrasonic-assisted extraction(75%increase),and supercritical CO2 extraction(173%increase).Mint flavonoids,within the concentration range of 1～100 μg/mL,significantly enhanced the survival rate of PC12 cells from 53.61%±0.64%to 78.49%±0.84%compared to the H2O2 group(P<0.001),indicating substantial alleviation of cellular oxidative injury.Conclusion:The study established an environmentally friendly,efficient,and reliable subcritical water extraction process for mint flavonoids.The extracted mint flavonoids demonstrated significant protective effects against H2O2-induced PC12 cell injury(P<0.01).
关键词
薄荷黄酮/亚临界水萃取/响应面法/细胞损伤/神经保护活性/工艺优化Key words
Mentha haplocalyx flavonoids/subcritical water extraction/response surface methodology/cell injury/neuroprotective activity/process optimization引用本文复制引用
基金项目
安徽省优秀青年人才支持计划(gxyq2020070)
出版年
2024
国家科技期刊平台
NSTL
万方数据