铜绿假单胞菌Gxun-2产铁载体发酵条件优化及其抑菌效果
Optimization of Siderophores Production and Its Antifungal Activity of Pseudomonas aeruginosa Gxun-2
李枢妍 1何艳丽 2宋超东 2黄夏至 2陆君铭 2李时勇 2张红岩 2申乃坤 2姜明国2
作者信息
- 1. 广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西南宁 530008;西北农林科技大学生命科学学院,陕西杨陵 712100
- 2. 广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西南宁 530008
- 折叠
摘要
鉴定铜绿假单胞菌(Pseudomonas aeruginosa)Gxun-2分泌铁载体类型,确定其最佳发酵工艺条件,同时考察铁载体发酵液对植物病原菌拮抗效果,为该菌株在农业中的应用提供理论依据.本试验先用铬天青(chrome azurol sulfonate,CAS)平板和全波段紫外光吸收法对P.aeruginosa Gxun-2产铁载体进行定性和定量分析,进一步通过单因素、Plackett-Burman及正交试验相结合的方法对其产铁载体培养基及培养条件进行了优化,最后利用平板对峙法测定产铁载体发酵液对香蕉枯萎病病原菌尖孢镰刀菌(Fusarium oxysporum f.sp.cubense,FOC)的拮抗效果.结果表明,菌株Gxun-2所产铁载体类型为异羟肟酸型,确定产铁载体最佳发酵工艺为:琥珀酸钠 2 g/L、甘油 10 g/L、硫酸铵 1 g/L,初始pH6.8、温度 35℃、接种量 1%(v/v)、装液量 140 mL/250 mL;在此条件下,菌株Gxun-2产铁载体活性单位(Su)达到 81.20%±0.24%,较优化前提高了 82.84%±0.82%,对FOC的抑菌率达70.60%±1.87%,较优化前提高了52.68%±4.23%.
Abstract
The aim of this study was to identify the type of siderophore secreted by Pseudomonas aeruginosa Gxun-2,determine the optimal fermentation conditions and investigate the antagonistic effect of fermentation broth on plant pathogens,so as to provide theoretical basis of the strain in agriculture.The chrome azurol sulfonate(CAS)plate and full-band ultraviolet light absorption method was to qualitatively and quantitatively analyze the siderophore production of P.aeruginosa Gxun-2.The iron-producing medium and culture conditions were optimized by the combined method of single-factor,Plackett-Burman and orthogonal tests.Finally,the plate confrontation method was used to assess the antagonistic effect of its fermentation brot on the pathogenic fungus,Fusarium oxysporum f.sp.cubense(FOC4).The results showed that the type of siderophore produced by strain Gxun-2 was isohydroxamic acid type,and the optimal fermentation medium and culture conditions were determined as follows:Sodium succinate 2 g/L,glycerol 10 g/L,ammonium sulfate 1 g/L,initial pH 6.8,temperature 35℃,inoculum 1.0%(v/v),and loading volume 140 mL/250 mL.Under these conditions,the siderophore activity unit(Su)of the Gxun-2 fermentation broth reached 81.20%±0.24%,which was 82.84%±0.82%higher than that before optimization,and the inhibition rate on FOC4 reached 70.60%±1.87%,which was 52.68%±4.23%higher than that before optimization.
关键词
铜绿假单胞菌/铁载体/发酵条件优化/尖孢镰刀菌/拮抗作用Key words
Pseudomonas aeruginosa/siderophores/optimization of fermentation/Fusarium oxysporum/antagonism引用本文复制引用
基金项目
广西科技重点研发计划(桂科AB21196019)
广西科技重点研发计划(桂科AB21220020)
国家自然科学基金(32160017)
国家自然科学基金(32060020)
广西壮族自治区研究生教育创新计划(YCSW2022256)
广西民族大学高层次人才引进项目(2018KJQD17)
广西民族大学相思湖青年学者创新团队项目(2017-6)
出版年
2024
国家科技期刊平台
NSTL
万方数据