螺旋藻肽亚铁螯合物的制备及结构表征
Preparation and Structural Characterization of Ferrous Chelates of Spirulina platensis Peptides
张雨 1李崴 1王丽娟 1李方巍 1曾名湧1
作者信息
- 1. 中国海洋大学三亚海洋研究院,海南三亚 572000;中国海洋大学食品科学与工程学院,山东青岛 266003
- 折叠
摘要
为了提高螺旋藻的高值化利用,本文以钝顶螺旋藻蛋白肽为实验原料,氯化亚铁为铁源,采用初步优化螺旋藻肽亚铁螯合物的螯合条件,并对螯合物通过分子量测定、氨基酸组成实验、荧光光谱、扫描电镜、量子化学计算进行结构表征.结果表明:初步合适的螯合条件为pH6、肽亚铁质量比 6:1、肽液浓度 1%、温度 60℃、时间 40 min,在此条件下螯合物得率为 44.13%.螺旋藻蛋白肽与亚铁盐螯合反应后产生了新结构,铁主要与谷氨酸和天冬氨酸的羧基、精氨酸的氨基、赖氨酸的胍基结合,且镶嵌在氨基酸电负性末端形成的空腔中,形成稳定的结构,证明了螯合物的形成.研究结果将为螺旋藻补铁剂的开发提供一定的理论依据.
Abstract
In order to improve the high-value utilization of Spirulina platensis,the chelating conditions of Spirulina platensis protein peptide were preliminatively optimized with ferrous chloride as the iron source.The chelates were characterized by molecular weight determination,amino acid composition experiment,fluorescence spectrum,scanning electron microscopy and quantum chemical calculation.The results showed that the suitable chelating conditions were pH6,mass ratio of ferrous peptide 6:1,concentration of peptide solution 1%,temperature 60℃and time 40 min.Under these conditions,the chelating yield was 44.13%.The peptide of Spirulina platensis produced a new structure after chelating with ferric salt.Iron was mainly combined with the carboxyl group of glutamic acid and aspartate,the amino group of arginine and the guanidine group of lysine,and was embedded in the cavity formed by the electronegative end of amino acid to form a stable structure,which proved the formation of chelates.The research results would provide a theoretical basis for the development of Spirulina platensis iron supplement.
关键词
螺旋藻蛋白肽/多肽亚铁螯合物/结构表征/量子化学计算Key words
Spirulina platensis protein peptide/polypeptide ferrous chelate/structural characterization/quantum chemical calculation引用本文复制引用
基金项目
海南省重点研发计划(ZDYF2021XDNY283)
出版年
2024
国家科技期刊平台
NSTL
万方数据