乳酸菌素对幽门螺杆菌感染性胃炎小鼠炎症和肠道菌群的影响
Effects of Lacidophilin on Inflammatory and Intestinal Microflora in Mice with Helicobacter pylori Induced Gastritis
程小英 1詹扬 1周衍安 2张静文 1李颖萌 1刘文君1
作者信息
- 1. 江中药业股份有限公司,江西南昌 330103
- 2. 南昌大学第一附属医院消化内科,江西南昌 330006
- 折叠
摘要
目的:探讨乳酸菌素对幽门螺杆菌(Helicobacter pylori,H.pylori)感染胃炎小鼠炎症和肠道菌群的影响.方法:通过口服H.pylori混悬液制备H.pylori感染小鼠模型.将H.pylori感染成功的小鼠随机分为模型组和乳酸菌素组,进行干预 4周.革兰氏染色、Warthin-Starry银染色和H.pylori免疫组化染色验证小鼠胃黏膜H.pylori的定植,HE染色观察小鼠胃黏膜组织病理学改变,免疫组化法测定小鼠胃黏膜 iNOS、IL-1β和3-Nitrotyrosine的表达,16S rRNA测序分析肠道菌群结构.结果:造模组小鼠H.pylori定植成功;乳酸菌素能改善H.pylori感染性胃炎小鼠胃组织病理形态;抑制胃黏膜中iNOS、IL-1β和 3-Nitrotyrosine的表达.相较于H.pylori感染组,乳酸菌素组小鼠的肠道菌群组成结构具有明显差异.乳酸菌素可通过调节厚壁菌门、拟杆菌门、放线菌门和疣微菌门的组成,增加肠道菌群的多样性.在属水平上,乳酸菌素可促进 g_norank_f_Muribaculaceae、阿克曼氏菌属(Akkermansia)和另枝菌属(Alistipes)等产生短链脂肪酸细菌的生长,抑制杜氏杆菌属(Dubosiella)等炎症相关细菌的增殖,改善肠道菌群组成结构.结论:乳酸菌素能够恢复由H.pylori感染破坏的肠道菌群结构,促进有益菌的增殖,降低炎症反应并缓解胃黏膜氧化损伤,对H.pylori感染性胃炎小鼠发挥保护作用.
Abstract
Objective:The aim of this study was to investigate the effect of lacidophilin on inflammation and intestinal microflora in H.pylori-induced gastritis mice.Methods:Established an H.pylori-infected mice model by orally adminis-tering an H.pylori suspension.The mice were randomly divided into a model group and a lacidophilin treatment group,which received treatment for four weeks.Gram staining,Warthin-Starry silver staining,and H.pylori immuno-histochemical staining were conducted to confirm the colonization of H.pylori in the gastric mucosa of mice.Hematoxylin and eosin(HE)staining was performed to observe any histopathological changes in the gastric mucosa of mice.Immu-nohistochemistry was carried out to observe the expression of iNOS,IL-1β,and 3-Nitrotyrosine in the gastric mucosa of mice.Additionally,the structure of intestinal microflora was analyzed using 16S rRNA sequencing.Results:The successful establishment of H.pylori-infected mice.Lacidophilin improved the stomach histopathological morphology in mice with H.pylori-induced gastritis and inhibited iNOS,IL-1β,and 3-Nitrotyrosine expression in the gastric mucosa.The com-position and structure of the gut microbiota among the lacidophilin group showed noticeable differences in comparison to the H.pylori-infected group.Lacidophilin augmented the diversity of intestinal microflora in H.pylori-infected mice by regulating the abundance of Firmicutes,Actinobacteriota,Bacteroidete,and Verrucomicrobiota.At the genus level,Lacidophilin resulted in significant growth stimulation of beneficial bacteria such as g_norank_f_Muribaculaceae,Akkermansia,and Alistipes,while suppressing the proliferation of inflammation-related bacteria such as Dubosiella,thereby improving the composition and structure of the gut microbiota.Conclusion:Lacidophilin has the potential to rebalance the composition and structure of gut microbiota that has been compromised by H.pylori infection.It may have a protective effect on mice with H.pylori-induced gastritis by promoting the growth of beneficial bacteria,reducing inflammatory reactions,and alleviating oxidative damage to the gastric mucosa.
关键词
乳酸菌素/幽门螺杆菌/胃炎/肠道菌群Key words
lacidophilin/Helicobacter pylori/gastritis/intestinal microflora引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据