Optimization of the Preparation of Lotus Seed Resistant Starch through Thermoacidophilic Type Ⅲ Pullulan Hydrolase Synergistic High-pressure Heat Treatments and Its Functional Characteristics
Optimization of the Preparation of Lotus Seed Resistant Starch through Thermoacidophilic Type Ⅲ Pullulan Hydrolase Synergistic High-pressure Heat Treatments and Its Functional Characteristics
In this study,lotus seed starch was used as the raw material for preparing RS3 resistant starch(EHP-LRS3)through thermoacidophilic type Ⅲ pullulan hydrolase synergistic high-pressure heat treatments.The parameters for this preparation process were optimized using a single-factor experimental design and response surface methodology.The morphological and crystal structures of EHP-LRS3 were observed and analyzed using scanning electron microscopy(SEM)and X-ray diffraction(XRD),respectively,and its pro-proliferative effect on Bifidobacterium longum was investigated.According to the results,the optimal conditions for preparing EHP-LRS3 with TK-PUL enzymatic hydrolysis temperature of 80 ℃ were as follows:Treatment of 35.32％lotus seed starch slurry(pH5.00)with 25.00 U/g(lotus seed starch)TK-PUL at 80 ℃ for 12.70 h,followed by high-pressure heat treatment of the mixture at 121 ℃ for 10 min and retrogradation at 4℃ for 24 h.An EHP-LRS3 yield of 58.46％was achieved under these optimal conditions.The SEM analysis revealed that the prepared EHP-LRS3 possessed an irregular ravine-like structure.The XRD analysis showed that the resistant starch had a B-type crystal structure,which differed from the A-type crystal structure of lotus seed starch.The pro-proliferative effect of EHP-LRS3 on B.longum was superior to that of RS3 resistant starch prepared using high-pressure heating alone(HP-LRS3).In summary,a high EHP-LRS3 yield was obtained using both TK-PUL and high-pressure heat treatments.The superior pro-proliferative effect of EHP-LRS3 on B.longum was verified.The results provide a theoretical basis for the high-value utilization of lotus seed starch.
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