富含γ-氨基丁酸的萌发红小豆对T2DM小鼠肠道菌群的影响
Effect of Rich GABA of Germinated Adzuki Bean on Intestinal Microflora in T2DM Mice
姜秀杰 1张家瑜 2李莹 2迟晓星 2孙东波 3曹冬梅 4张东杰4
作者信息
- 1. 黑龙江八一农垦大学食品学院,黑龙江大庆 163319;农业农村部农产品及加工品质量监督检验测试中心,黑龙江大庆 163319
- 2. 黑龙江八一农垦大学食品学院,黑龙江大庆 163319
- 3. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
- 4. 黑龙江八一农垦大学食品学院,黑龙江大庆 163319;黑龙江八一农垦大学国家杂粮工程技术研究中心,黑龙江大庆 163319
- 折叠
摘要
为了研究富含GABA萌发红小豆对2型糖尿病(Diabetes Mellitus Type 2,T2DM)小鼠血糖水平及肠道菌群的影响,采用C57BL/6J小鼠为研究对象,通过高脂膳食+链脲菌素(Streptozocin,STZ)注射构建T2DM模型,选择不同剂量的富含GABA萌发红小豆对T2DM小鼠连续膳食干预6周,并利用16S rRNA测序技术对T2DM小鼠盲肠内容物的菌群结构和分布进行鉴定.结果显示:不同剂量富含GABA红小豆膳食干预可使T2DM小鼠FBG值明显下降,其中高剂量富含GABA红小豆(TF3)组FBG值为8.36±0.78 mmol/L,相比模型组(M)下降54.09％,干预效果最好.此外,TF3膳食可引起T2DM小鼠肠道菌群丰度发生显著(P＜0.05)改变,门水平上Firmicutes丰度为35.96％,比M模型组下降53.17％,并可显著上调Bacteroidetes、Verrucomicrobia的菌群丰度(P＜0.05).表明TF3膳食改善糖脂代谢与Bacteroidetes、Verrucomicrobia优势菌丰度呈正相关,暗示高剂量富含GABA红小豆膳食可通过增加有益菌来缓解T2DM小鼠高血糖症状,为进一步解释T2DM与肠道菌群的关系提供参考.
Abstract
In order to study the effects of GABA-rich germinated adzuki bean on blood glucose level and intestinal flora in T2DM mice.C57BL/6J mice were used as subjects to construct T2DM model by high fat diet+STZ injection.Different doses of GABA-rich germinated adzuki bean were used to treat T2DM mice for 6 weeks and 16S rRNA sequencing technique was used to identify the microbial structure and distribution in the cecal contents of T2DM mice.The results showed that different doses of GABA-rich adzuki bean dietary intervention could significantly reduce the FBG value of T2DM mice,and the FBG value of high dose GABA-rich adzuki bean(TF3)group was 8.36±0.78 mmol/L,which was 54.09％,lower than that of model group(M).In addition,TF3 diet induced significant changes in intestinal flora abundance in T2DM mice.Firmicutes abundance at gate level was 35.96％,which decreased by 53.17％compared with M model group,and Bacteroidetes and Verrucomicrobia abundance were significantly up-regulated.In summary,the improvement of glucose and lipid metabolism by TF3 diet was significantly correlated with the abundance of dominant bacteria in Bacteroidetes and Verrucomicrobia,suggesting that high dose of GABA-rich adzuki bean diet could alleviate hyperglycemia symptoms in T2DM mice through beneficial bacteria,which provided reference for further explaining the relationship between T2DM and intestinal flora.
关键词
红小豆/萌发/γ-氨基丁酸/2型糖尿病/肠道菌群Key words
adzuki bean/germinating/GABA/T2DM/intestinal microflora引用本文复制引用
基金项目
黑龙江省博士后资助经费项目(LBH-Z23241)
黑龙江省"百千万"工程科技重大专项(2021ZX12B06)
黑龙江省自然科学基金(SS2022C001)
学成、引进人才科研启动基金(XYB202210)
黑龙江省杂粮生产与加工优势特色学科建设项目(2022-78)
黑龙江八一农垦大学"三纵"基础培育项目(ZRCPY202122)
出版年
2024
国家科技期刊平台
NSTL
万方数据