复合益生菌后生元的功效及有益代谢产物的研究
Efficacy and Beneficial Metabolites of Postbiotics Derived from Composite Probiotics
钟智 1徐维那 1郭帅 1路镜达 1邱景良 1王记成1
作者信息
- 1. 内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特 010018
- 折叠
摘要
为了研究复合益生菌后生元的功能和作用机制,本研究以副干酪乳酪杆菌ProSci-101和青春双歧杆菌B8589复合发酵的后生元为研究对象,利用代谢组学技术分析了复合益生菌后生元的有益代谢产物.结果显示,复合益生菌后生元的抑菌能力和抗氧化能力显著(P＜0.05)强于单菌株发酵后生元.代谢组学分析结果显示,复合益生菌后生元与ProSci-101和B8589单菌株发酵的后生元相比,分别存在159和161个差异代谢产物,差异代谢通路主要为氨基酸和甘油磷脂代谢等.复合益生菌后生元中富集了生物碱、黄酮、有机酸等有益代谢物.研究为复合益生菌后生元的开发及应用提供了理论依据.
Abstract
In order to study the functions and mechanisms of the postbiotics derived from composite probiotics,this study focused on the postbiotics produced by the co fermentation of Lacticaseibacillus paracasei ProSci-101 and Bifidobacterium adolescentis B8589.Metabolomics techniques were used to analyze the beneficial metabolites of the postbiotics.The results showed that the antibacterial and antioxidant abilities of the postbiotics derived from composite probiotics were signifi-cantly(P＜0.05)stronger than those of single strain fermentation postbiotics.Metabolomics analysis showed that there were 159 and 161 differential metabolites in the postbiotics derived from composite probiotics compared to the postbiotics derived from ProSci-101 and B8589 single strains,respectively.The differential metabolic pathways were mainly amino acid and glycerophospholipid metabolism.postbiotics derived from composite probiotics are enriched with beneficial metabolites such as alkaloids,flavonoids,and organic acids.The study provides a theoretical basis for the development and application of postbiotics derived from composite probiotics.
关键词
益生菌/后生元/抑菌/抗氧化/代谢产物Key words
probiotics/postbiotics/antibacterial/antioxidant/metabolites引用本文复制引用
基金项目
国家自然科学基金(32260572)
内蒙古自然科学基金重点项目(2023ZD17)
内蒙古自治区高等学校青年科技英才支持项目(NJYT24055)
出版年
2024
国家科技期刊平台
NSTL
万方数据