金花茶多酚制备、成分分析及其体内外抗氧化活性分析
Preparation and Component Analysis of Camellia nitidissima Chi Polyphenols and Their Antioxidant Activites in Vivo and in Vitro
许嘉文 1孙庆 1阿丽娅·阿布杜热依木 1唐伟 2阮琳 2孙秀岩 1解军波1
作者信息
- 1. 天津中医药大学中药学院,天津 301617
- 2. 广西金花茶产业工程研究院,广西防城港 538025
- 折叠
摘要
为了探究金花茶多酚最佳提取工艺条件及纯化后总多酚成分及体内外抗氧化活性分析.本研究以金花茶为原料,在单因素实验的基础上,设计响应面试验,筛选超声功率、提取温度、乙醇浓度和料液比最佳提取参数.采用Lx-8型大孔树脂对提取物进行纯化,并以高效液相色谱法对5种多酚单体化合物进行定性定量分析,随后对提取物进行体内外抗氧化实验.结果表明,最优工艺条件为超声功率290 W,提取温度35 ℃,乙醇浓度55％,料液比1∶30g/mL,在此条件下,金花茶多酚得率为3.53％,茶多酚纯度为63.73％.5种多酚单体化合物没食子酸、绿原酸、表儿茶素、芦丁、鞣花酸含量分别为0.47、11.18、59.03、18.34、16.41 mg/g.体外抗氧化实验结果表明,金花茶花多酚能够有效清除DPPH自由基、ABTS+自由基和超氧阴离子自由基,其IC50值分别为0.38、0.16、0.39 mg/mL.此外,金花茶多酚还能够通过显著提高线虫体内SOD酶活力及降低MDA含量,有效增强秀丽隐杆线虫对氧化损伤的抵抗力.
Abstract
This study aimed to investigate the optimal extraction process for polyphenols from Camellia nitidissima Chi and to analyze the total polyphenol composition and antioxidant activities both in vivo and in vitro post-purification.Camellia nitidissima Chi was the raw material and a response surface methodology based on single factor experiments was designed to screen the optimal extraction parameters including ultrasound power,extraction temperature,ethanol concentration and solid-liquid ratio.The extract was purified using Lx-8 macroporous resin,and five specific polyphenolic monomers were qualitatively and quantitatively analyzed using high-performance liquid chromatography(HPLC).Subsequently,in vivo and in vitro antioxidant experiments were conducted on the extract.The results showed that the optimal conditions were an ultrasonic power of 290 W,an extraction temperature of 35 ℃,an ethanol concentration of 55％,and a material-liquid ratio of 1∶30 g/mL.Under these conditions,the yield of polyphenols from Camellia nitidissima Chi was 3.53％,the purity was 63.73％.The content of five polyphenolic monomer compounds,namely gallic acid,chlorogenic acid,epicatechin,rutin,and ellagic acid was 0.47,11.18,59.03,18.34,and 16.41 mg/g,respectively.In addition,the polyphenols extracted from Camellia nitidissima Chi demonstrated a significant ability to boost oxidative damage resistance in C.elegans.This was evidenced by a marked increase in SOD(superoxide dismutase)enzyme activity and a decrease in MDA(malondialdehyde)levels within the C.elegans specimens.
关键词
金花茶多酚/超声辅助提取/响应面法/成分分析/秀丽隐杆线虫/抗氧化损伤Key words
Camellia nitidissima Chi polyphenols/ultrasound-assisted extraction/response surface methodology/compositional analysis/Caenorhabditis elegans/antioxidant damage引用本文复制引用
基金项目
天津市"131"创新型人才项目(201927)
出版年
2024
国家科技期刊平台
NSTL
万方数据