不同灵芝菌丝体水提物对乙醇诱导LO2肝细胞损伤的保护作用
Protective Effects of Water Extracts from Different Ganoderma lucidum Mycelium on Ethanol-induced Hepatocyte Injury in LO2 Cells
陈亚平 1刘利平 2冯杰 2刘艳芳 2唐传红 2颜梦秋 2张劲松 2韩伟3
作者信息
- 1. 华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237;上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室 上海 201403
- 2. 上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室 上海 201403
- 3. 华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237
- 折叠
摘要
为了探讨不同灵芝菌丝体水提物对乙醇诱导LO2肝细胞损伤的保护作用,本文分析了 7种不同灵芝菌株发酵菌丝体水提物对乙醇诱导LO2细胞损伤的保护作用.利用乙醇诱导LO2细胞建立体外肝损伤模型,并以细胞存活率、谷丙转氨酶(Alanine Aminotransferase,ALT)、谷草转氨酶(Aspartate Aminotransferase,AST)、活性氧(Reactive Oxygen Species,ROS)、超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽(Glutathione,GSH)和丙二醛(Malondiadehyde,MDA)水平为指标考察灵芝菌丝体水提物对乙醇诱导LO2细胞损伤的保护效果.结果表明,7种灵芝菌株的菌丝体水提物均具有不同程度的保护作用.综合比较,G0119与G0154菌丝体的水提物作用效果较优,相较模型组,LO2细胞存活率显著提高(P＜0.05),细胞培养液中的ALT、AST活力显著降低(P＜0.05),细胞内ROS水平显著降低(P＜0.05),SOD活力、GSH含量显著升高(P＜0.05),MDA含量显著降低(P＜0.05).经相关性分析,发现水提物中多糖组分2的分子量大小与其保肝活性呈正相关,即分子量越大,活性越强.综上,灵芝菌丝体水提物对乙醇诱导的体外肝损伤起到保护作用,并且G0119与G0154菌株的作用效果较优,其保肝作用与提取物中多糖组分的分子量大小相关.
Abstract
To explore the protective effects of water extracts from different Ganoderma lucidum(G.lucidum)mycelium on ethanol-induced hepatocyte injury in LO2 cells.The effects of water extracts obtained from different G.lucidum mycelium against ethanol-induced damage to LO2 cells were examined in this research.Cell survival rate,alanine aminotransferase(ALT),aspartate aminotransferase(AST),reactive oxygen species(ROS),superoxide dismutase(SOD),glutathione(GSH)and malondialdehyde(MDA)levels were measured in a liver injury model employing ethanol-induced LO2 cells,to assess the preventive effects of G.lucidum mycelium water extracts against ethanol-induced damage.The results showed that water extracts from the mycelium of all seven strains exhibited varying degrees of protective effects.Among them,the water extract from strains G0119 and G0154 had the best protective effect.Compared with the model group,the intervention of water extracts significantly increased LO2 cell survival(P＜0.05),while decreased ALT and AST levels in the cell culture medium,respectively(P＜0.05).Furthermore,the intracellular ROS level significantly decreased(P＜0.05),while the activity of SOD and the content of GSH significantly increased(P＜0.05),and the MDA content significantly decreased compared to the model group(P＜0.05).Correlation analysis revealed a positive correlation between the molecular weight of polysaccharide component 2 in the water extract and its hepatoprotective activity,with higher molecular weight correlating with stronger activity.The results indicated that the water extract of G.lucidum mycelium exhibited a protective effect against ethanol-inducedinvitro liver damage.Moreover,the effectiveness of the G0119 and G0154 strains were superior,and its hepatoprotective effect was correlated with the molecular weight of polysaccharide components in the extract.
关键词
灵芝/菌丝体/水提物/乙醇性肝损伤/保肝作用Key words
Ganoderma lucidum/mycelium/water extract/ethanol-induced liver injury/hepatoprotective effects引用本文复制引用
基金项目
上海市现代农业产业技术体系建设项目(沪农科产字2023第9号)
上海市农科院卓越团队建设计划(沪农科卓2022003)
出版年
2024
国家科技期刊平台
NSTL
万方数据