外源糖处理对花生芽菜中γ-氨基丁酸富集的影响
Effects of Exogenous Sugar Treatment on Enrichment of γ-Aminobutyric Acid in Peanut Sprouts
赵柯馨 1蓝威豪 1杜佳丽 1陈静文 1范子欣 1韦沁伶 1王鸿飞 1许凤1
作者信息
- 1. 宁波大学食品与药学学院,浙江宁波 315211
- 折叠
摘要
为探究糖处理对花生芽菜中γ-氨基丁酸(γ-aminobutyric acid,GABA)富集的影响,本文筛选了不同类型外源糖(甘露糖、蔗糖和葡萄糖)的最佳施用浓度,探究了其对花生种子发芽过程中GABA以及GABA合成代谢有关物质的影响并对其可能的作用机制进行了研究.结果表明,甘露糖、蔗糖和葡萄糖处理可以显著(P＜0.05)提高花生芽菜中GABA的含量,且谷氦酸脱羧酶(Glutamate decarboxylase,GAD)、二胺氧化酶(Diamine oxidase,DAO)、多胺氧化酶(Polyamine oxidase,PAO)活性,以及谷氨酸、腐胺、精胺和亚精胺等的含量均发生了显著(P＜0.05)变化.同时,根据测定结果可知,不同外源糖处理均可在不同程度上提升花生芽菜中抗坏血酸、蛋白质和白藜芦醇的含量.花生芽菜中GABA可通过外源糖处理富集,其影响机制可能为GABA支路和多胺降解途径.
Abstract
In order to explore the influence of sugar treatment on the enrichment of γ-aminobutyric acid(GABA)in peanut sprouts,this paper selected the optimal application concentration of different types of exogenous sugars(mannose,sucrose and glucose).The effects of GABA on the germination of peanut seeds and the related substances of GABA anabolic metabolism were investigated and the possible mechanism of action was studied.The results showed that mannose,sucrose and glucose treatment could significantly(P＜0.05)increase the content of GABA in peanut sprouts,and the activity of glutamate decarboxylase(GAD),diamine oxidase(DAO),polyamine oxidase(PAO)and the contents of glutamic acid,putrescine,spermidine and spermidine were significantly changed in oxidase(P＜0.05).At the same time,according to the test results,different exogenous sugar treatments could increase the contents of ascorbic acid,protein and resveratrol in peanut sprouts to different degrees.GABA in peanut sprouts can be enriched by exogenous sugar treatment,and its influence mechanism may be GABA branch and polyamine degradation pathway.
关键词
糖处理/花生芽菜/γ-氨基丁酸/GABA支路/多胺降解途径Key words
sugar treatment/peanut sprouts/γ-aminobutyric acid/GABA pathway/polyamine degradation pathway引用本文复制引用
基金项目
国家自然科学基金(32372393)
国家自然科学基金(31772030)
宁波市项目(2022J122)
宁波市公益项目(2023S191)
宁波市公益项目(2022S154)
出版年
2024
国家科技期刊平台
NSTL
万方数据