鲍鱼脏器碱提多糖制备工艺及对L929细胞H2O2氧化损伤的修复活性
Preparation Process of Alkali-extracted Polysaccharides from Abalone Viscera and Its Repair Activity against H2O2 Oxidative Damage in L929 Cells
吴启赐 1林志超 1潘晓明 2薛钰 1黄家福 1潘裕添1
作者信息
- 1. 闽南师范大学菌物产业工程技术中心,福建漳州 363000
- 2. 萌得尔(厦门)生物科技有限公司,福建厦门 361000
- 折叠
摘要
目的:优化鲍鱼脏器碱提多糖(Alkali-extracted abalone viscera polysaccharides,Aavp)提取工艺,并研究纯化Aavp对L929细胞H2O2氧化损伤的预防和修复作用,以期为鲍鱼脏器碱提多糖的开发及应用提供参考.方法:通过热碱提醇沉法获得鲍鱼脏器碱提粗多糖,并利用响应面法优化提取工艺,最后分析纯化Aavp对L929细胞H2O2氧化损伤的预防和修复能力.结果:鲍鱼脏器碱提粗多糖最佳提取工艺条件为:料液比1∶150g/mL,提取时间2h,提取温度70 ℃,在此条件下,鲍鱼脏器碱提粗多糖得率为8.57％.在L929细胞H2O2氧化损伤预防试验中,不同剂量的纯化Aavp(20、50和100 µg/mL)对L929细胞氧化损伤均具有极显著的预防作用(P＜0.00l),但没有表现出剂量效应,其中中剂量组(50μg/mL)的细胞存活率最高(71.94％±3.08％),略优于阳性对照的维生素E组,但无显著性差异.在修复试验中,不同剂量的纯化Aavp(20、50和100 μg/mL)对L929细胞氧化损伤均具有极显著的修复效果(P＜0.001),并呈现剂量效应,而高剂量组(100μg/mL)的细胞存活率最高(90.93％±1.17％),略高于阳性对照的维生素E组(87.96％±3.05％).结果表明鲍鱼脏器碱提多糖对L929细胞H2O2氧化损伤具有出较好的预防和修复作用.结论:鲍鱼脏器碱提多糖具有一定的L929细胞H2O2氧化损伤修复能力.
Abstract
Objective:The extraction process of alkali-extracted abalone viscera polysaccharides(Aavp)was optimized,and the preventive and repair effects of purified Aavp on H2O2 oxidative damage on L929 cells were studied to provide a reference for the development and application of Aavps.Methods:Crude Aavp was obtained by hot alkali extraction followed by alcohol precipitation,and its extraction process was optimized by the response surface method.The preventive and repair abilities of H2O2 oxidative damage on L929 cells were analyzed.Resulst:The optimum extraction conditions of crude Aavps were as follows:Solid-liquid ratio of 1∶150 g/mL,extraction time of 2 h,and extraction temperature of 70 ℃.Under these optimal conditions,the yield of crude Aavp was 8.57％.In the prevention test of H2O2 oxidative damage on L929 cells,different doses of purified Aavp(20,50 and 100 μg/mL)had a significant preventive effect on L929 cell oxidative damage(P＜0.001)but did not show a dose effect.The cell survival rate of the middle dose group(50 μg/mL)was the highest(71.94％±3.08％),which was slightly better than the positive control vitamin E group but not significant.In the repair test,different doses of purified Aavp(20,50 and 100 μg/mL)had a significant repair effect on the oxidative damage of L929 cells(P＜0.001)and showed a dose effect.In contrast,the cell survival rate of the high-dose group(100 μg/mL)was the highest(90.93％±1.17％),slightly higher than that of the positive control vitamin E group(87.96％±3.05％).The results showed that purified Aavp showed an excellent preventive and repair effect on H2O2 oxidative damage in L929 cells.Conclusion:Aavp has an ability to repair H2O2 oxidative damage of L929 cells.
关键词
鲍鱼脏器/碱提多糖/响应面优化/氧化损伤Key words
abalone viscera/alkali-extracted polysaccharide/response surface optimization/oxidative damage引用本文复制引用
基金项目
福建省自然科学基金面上项目(2020J01822)
福建省科技厅对外合作项目(2022I0022)
国家科技项目(2021L3027)
闽南师范大学培育项目(MSPY2022002)
闽南师范大学培育项目(MSPY202101)
出版年
2024
国家科技期刊平台
NSTL
万方数据