猴头菌产酚类物质液态培养条件优化及对乳酸菌生长的影响
Optimization of Liquid Culture Conditions for Phenolic Substances Produced by Hericium erinaceus and Its Influence on the Growth of Lactic Acid Bacteria
马永强 1梁珊珊 1马宏良 1王瑞琦 1王植朔 1那治国 1徐伟1
作者信息
- 1. 哈尔滨商业大学食品工程学院,黑龙江省谷物食品与谷物资源综合加工重点实验室,黑龙江哈尔滨 150028
- 折叠
摘要
目的:用猴头菌纯菌株进行液态菌丝培养,优化培养基以提高猴头菌丝体酚类提取物的含量,分析猴头菌丝体酚类提取物的组成,并研究猴头菌丝体酚类提取物对乳酸菌的影响.方法:采用正交试验设计,研究对液态菌丝中总酚含量的影响,并用HPD-826大孔吸附树脂对其进行纯化,再以液质联用(High performance liquid chromatography-mass spectrometry,HPLC-MS)检测分析其组成.最后,将纯化物添加到乳酸菌培养基中,检测其对乳酸菌活菌数与产酸能力的影响.结果:在碳源马铃薯14％、氮源蛋白胨2％、无机盐MgSO4 0.3％时,猴头菌丝体总酚含量达到0.783 mg/g;从猴头菌丝体酚类提取物中鉴定出16个单体酚,占比88.27％,主要单体酚为没食子酸-O-葡萄糖苷、对香豆酸-O-没食子酰葡萄糖苷、阿魏酸、儿茶素苄硫醚、没食子酸和咖啡酸.在干酪乳杆菌(Lactobacilluscasei)、嗜酸乳杆菌(Lactobacillus acidophilus)、肠膜串珠菌(Streptococcus intestinalis)的 MRS培养基中加入猴头菌丝体酚类提取溶液,可以使3种乳酸菌活菌数均增加.结论:猴头菌丝体酚类提取物对乳酸菌的生长具有促生作用,为功能食品提供了新资源原料.
Abstract
Objetive:Culturing liquid hyphae with Hericium erinaceus pure strain.The medium was optimized to increase the content of Hericium erinaceus mycelium phenolic extracts.The composition of Hericium erinaceus mycelium phenolic extracts was analyzed,and the effects of phenolic extracts from Hericium erinaceus mycelium on lactic acid bacteria were also studied.Methods:Orthogonal design was used to study the effect on the total phenol content in liquid mycelium,and HPD-826 macroporous adsorption resin was used to purify it,and then HPLC-MS was used to determine its composition.Finally,adding the purified product to the lactic acid bacteria culture mediumm,and detected the effect on the number of viable lactic acid bacteria and its acid-production ability.Results:When the carbon source potato was 14％,nitrogen source peptone was 2％and inorganic salt MgSO4 was 0.3％,the total phenol content of Hericium erinaceus mycelium reached 0.783 mg/g.Sixteen monomeric phenols were identified from the phenolic extract of Hericium erinaceus mycelium,accounting for 88.27％.The main monomeric phenols were gallic acid-O-glucoside,p-coumaric acid-O-gallic acid glucoside,ferulic acid,catechin benzyl sulfide,gallic acid and caffeic acid.Adding phenol extraction solution from Hericium erinaceus mycelium to MRS medium of Lactobacilluscasei,Lactobacillus acidophilus and Streptococcus intestinalis could increase the number of viable bacteria of three kinds of lactic acid bacteria.Conclusion:Phenolic extract from Hericium erinaceus mycelium can promote the growth of lactic acid bacteria and provide new resource raw materials for functional food.
关键词
液态培养猴头菌丝体/酚类提取物/液质联用/乳酸菌Key words
liquid culture of Hericium erinaceus mycelium/phenolic extract/HPLC-MS/lactic acid bacteria引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据