大金发藓乙醇提取物对4种植物病原真菌的抑菌活性
Antifungal Activity of Ethanol Extract of Polytrichum commune Hedw.Against Four Plant Pathogenic Fungi
申海玉 1付静 1韩超 2刘颂赞 2刘郡瑞 2房于凡 2安博涵 2王梦婷 2王彬锋2
作者信息
- 1. 邯郸学院生命科学与工程学院,河北邯郸 056005;河北省高校冀南太行山区野生资源植物应用技术研发中心,河北邯郸 056005
- 2. 邯郸学院生命科学与工程学院,河北邯郸 056005
- 折叠
摘要
为给植物源杀菌剂的研发提供备选材料,采用菌丝生长速率法和孢子萌发法测定大金发藓(Polytrichum commune Hedw.)茎叶和假根的乙醇提取物对4种植物病原真菌灰葡萄孢菌(Botrytis cinerea)、茄病镰刀菌甘薯专化型(Fusarium solani f.sp.batatas)、茄病镰刀菌(Fusarium solani)和细极链格孢菌(Alternaria tenuissima)菌丝生长和孢子萌发的抑制率及其抑制中浓度(EC50),对抑菌活性进行分析.结果表明,浓度在1～8 mg/mL时,大金发藓茎叶醇提液和假根醇提液对4种植物病原真菌产生极显著抑菌效果(P＜0.01),且随提取液使用浓度增加,对4种病原菌菌丝生长和分生孢子萌发的抑制率增强;在相同浓度下,茎叶醇提液对4种植物病原真菌的抑制效果显著优于假根醇提液(P＜0.05);茄病镰刀菌(甘薯)对大金发藓茎叶和假根醇提液的抑菌作用最敏感,EC50值分别仅为0.42和0.35 mg/mL.综合而言,大金发藓茎叶和假根醇提液对4种植物病原真菌存在较强抑菌效果,茎叶醇提液抑菌效果优于假根,二者在植物源杀菌剂的研发中具有一定的应用潜力.
Abstract
In order to exploit new botanic fungicide,the antifungal activities of the ethanol extracts of stem-leaf and rhizoid of P.commune were studied by the mycelia growth rate method and the spore germination method based on the determination of their mycelia growth inhibition rates,spore germination inhibition rates and median inhibition concentration values(EC50)on B.cinerea,F.solani f.sp.batatas,F.solani and A.brassicicola.The results showed that the antifungal activities of the ethanol extracts of P.commune against the four plant pathogenic fungi were extremely significant(P＜0.01)in the concentration range of 1 to 8 mg/mL and the antifungal activities were proportional to the concentration of ethanol extracts of P.commune.Furthermore,under the same concentration,the antifungal activity of the alcohol extracts of the stem-leaf of P.commune against the four plant pathogenic fungi was significantly higher than that of the rhizoid of P.commune(P＜0.05).Finally,among the four plant pathogenic fungi,F.solani f.sp.batatas was the most sensitive to the antifungal activity of the ethanol extracts of the stem-leaf and rhizoid of P.commune,with the EC50 values of stem-leaf and rhizoid only 0.42 and 0.35 mg/mL,respectively.In summary,the ethanol extracts of the stem-leaf and rhizoid of P.commune had strong antifungal activities against the four plant pathogenic fungi,and the antifungal activity of the stem-leaf was better than that of the rhizoid.Both of them had certain potential application in the development of new botanical fungicides.
关键词
大金发藓/乙醇提取物/灰葡萄孢菌/茄病镰刀菌/细极链格孢菌/抑菌效果Key words
Polytrichum commune Hedw./ethanol extract/Botrytis cinerea/Fusarium solani/Alternaria tenuissima/antifungal activity引用本文复制引用
基金项目
国家自然科学基金资助项目(32071501)
河北省科技厅农业科技园区建设专项(20536404D)
邯郸市科技研发项目(19422017001-16)
邯郸学院校级项目(XZ2022103)
大学生创新创业项目(S202210103039)
出版年
2024
国家科技期刊平台
NSTL
万方数据