刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用
Ameliorating Effect of Polyphenol Extract of Rosa roxburghii on Obese Mice Induced by High Fat Diet
罗婧文 1张欣 2王辉 1龙明秀 3陈朝军 1陈中爱 1王梅1
作者信息
- 1. 贵州省农科院食品加工研究所,贵州贵阳 550025;贵州省农业生物技术重点实验室,贵州贵阳 550025
- 2. 贵州大学酿酒与食品工程学院,贵州贵阳 550025
- 3. 贵州省现代农业发展研究所,贵州贵阳 550025
- 折叠
摘要
目的:为了研究刺梨多酚提取物对高脂饮食诱导的肥胖小鼠的改善作用.方法:分别以多酚含量400 mg/kg BW/d的剂量对高脂饮食小鼠进行干预.连续灌胃4周,测定小鼠体重、肝脏指数、白色脂肪及肝脏组织形态学变化、血清生化指标、脏器抗氧化能力和肠道屏障.结果:与CON组相比,MODEL组胰岛素水平(Insulln,INS)和炎症因子水平均显著(P＜0.05,P＜0.01)提高,血脂四项中高密度脂肪(High-density lipoprotein-cholesterol,HDL-C)水平显著降低(P＜0.01),总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)和低密度脂肪(Low-density lipoprolesterol,LDL-C)水平显著升高(P＜0.01);抗氧化物中超氧化物歧化酶(Superoxide Dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GPx)活力显著下降(P＜0.01),肠道屏障蛋白表达水平及短链脂肪酸(Short-chain fatty acids,SCFAs)水平均显著降低(P＜0.01);与MODEL相比,多酚干预组(RF、RP组)炎症因子和氧化应激水平显著降低(P＜0.05,P＜0.01),RF组抗氧化物SOD、GPx活力显著提升(P＜0.01),RF组和RP组肠道内ZO-1、Claduin-1和SCFAs水平均发生显著逆转(P＜0.01).结论:刺梨多酚提取物能有效缓解高脂饮食诱导的肥胖小鼠体内代谢紊乱,减缓体重增长,保护脂肪和肝脏组织细胞形态变化,缓解肥胖小鼠体内炎症和氧化应激水平,增强肠道屏障功能,且RF效果优于RP.
Abstract
Objective:To investigate the beneficial effects of phenolic extracts of Rosa roxburghii Tratt.on obese mice induced by a high-fat diet.Methods:The intervention involved administering polyphenol extract at a dose of 400 mg/kg body weight per day to mice on a high-fat diet.The mice were orally gavaged continuously for 4 weeks,and various parameters such as body weight,liver index,histomorphological changes in white fat and liver tissues,serum biochemical markers,organ antioxidant capacity,and intestinal barrier function were examined.Results:In comparison to control group(CON),the MODEL group(MODEL)exhibited significantly(P＜0.05,P＜0.01)increased the level of insulin and inflammatory factors.The levels of high-density lipoprotein cholesterol(HDL-C)decreased in the four blood lipids,while the levels of total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)significantly increased(P＜0.01).The activities of superoxide dismutase(SOD)and glutathione peroxidase(GPx)in antioxidants were significantly reduced(P＜0.01),the expression levels of intestinal barrier proteins and short chain fatty acids(SCFAs)were significantly reduced(P＜0.01).In comparison to MODEL group,the polyphenol intervention groups(RF and RP)showed significantly decreased levels of inflammatory factors and oxidative stress(P＜0.05,P＜0.01),The activity of antioxidant SOD and GPx in the RF group was significantly increased,and the levels of ZO-1,Claduin-1,and SCFAs in the gut of both the RF and RP groups were significantly reversed(P＜0.01).Conclusion:Prickly pear polyphenol extract effectively alleviated metabolic disorders in high-fat diet-induced obese mice in vivo,resulting in reduced body weight gain,protection against cellular changes in adipose and hepatic tissues,alleviation of inflammation and oxidative stress,and enhanced intestinal barrier function.Notably,the RF group showed a superior effect to the RP group.
关键词
刺梨多酚提取物/肥胖小鼠/血脂/炎症因子/短链脂肪酸Key words
phenolic extracts of Rosa roxburghii Tratt./obese mice/lipids/inflammatory factors/short-chain fatty acids引用本文复制引用
基金项目
贵州省科技厅科技支撑项目(黔科合支撑[2022]一般149)
贵州省科技厅科技支撑项目(黔科合支撑[2021]一般116)
贵州省科技厅科技支撑项目(黔科合支撑[2020]1Y155号)
贵州省科技厅2023年度贵州省基础研究计划(黔科合基础-ZK[2023]一般182)
出版年
2024
国家科技期刊平台
NSTL
万方数据