超声辅助碱法提取无籽刺梨结合多酚工艺优化及其抗氧化活性分析
Optimization of Ultrasound-assisted Alkali Extraction of Bound Polyphenol from Rosa sterilis and Its Antioxidant Activity
秦毅臻 1何洋 1冯怡婷 1范方宇2
作者信息
- 1. 西南林业大学生命科学学院,云南昆明 650224
- 2. 西南林业大学生命科学学院,云南昆明 650224;云南省森林灾害预警与控制实验室,云南昆明 650224
- 折叠
摘要
为探究无籽刺梨在药食同源领域的应用及开发,以无籽刺梨为原料,在单因素实验的基础上,采用Box-Behnken法优化无籽刺梨结合多酚的提取工艺,以无籽刺梨游离多酚和VC为对照,研究结合多酚对DPPH·、ABTS+·、·OH的清除率和总还原力.结果表明:无籽剌梨结合多酚最优提取工艺为液料比24mL/g、碱液浓度8mol/L、超声时间47 min、超声功率250 W,该条件下结合多酚提取量为135.73 mg GAE/g,与回归模型预测值(135.77 mg GAE/g)接近,模型拟合极好.所得结合多酚对DPPH·、ABTS+·的IC50值分别为0.0599、0.0121 mg/mL,显著(P＜0.05)优于游离多酚(0.168、0.0395 mg/mL).在浓度0.02～0.1 mg/mL范围内,结合多酚·OH清除能力较强,与VC无显著差异(P＞0.05).结合多酚总还原力优于游离多酚,2种多酚的总还原力与溶液浓度呈线性依赖关系.提取的结合多酚可作为天然抗氧化剂使用,为无籽刺梨加工利用提供理论依据.
Abstract
In order to investigate the application and development of Rosa sterilis in the field of drug-food homology,the Box-Behnken method was used to optimize the extraction process of bound polyphenols from Rosa sterilis based on the single factor experiment.The study also examined the clearance rate of DPPH·,ABTS+·,and·OH and total reducing power of bound polyphenols and free polyphenols from Rosa sterilis,with Vc as controls.The results showed that the optimal extraction conditions for the bound polyphenols of Rosa sterilis were a liquid-to-solid ratio of 24 mL/g,a lye concentration of 8 mol/L,an ultrasonic time of 47 min,and an ultrasonic power of 250 W.Under these conditions,the amount of extracted bound polyphenols was 135.73 mg GAE/g,which was close to the predicted value of the regression model(135.77 mg GAE/g).The model fit was excellent.The IC50 values of the bound polyphenols to DPPH and ABTS+.were 0.0599 and 0.0121 mg/mL,respectively.These values were significantly(P＜0.05)lower than those of the free polyphenols(0.168,0.0395 mg/mL).In the concentration range of 0.02～0.1 mg/mL,the scavenging ability of bound polyphenols on·OH was strong,which was not significantly different from that of VC(P＞0.05).The total reducing power of the bound polyphenols was higher than that of the free polyphenols.Additionally,the total reducing power of both polyphenols was significantly influenced by the concentration of the solution.The extracted bound polyphenols can be used as natural antioxidants,providing a theoretical basis for the processing and utilization of Rosa sterilis.
关键词
无籽刺梨/结合多酚/抗氧化活性/超声辅助碱法Key words
Rosa sterilis/bound polyphenol/antioxidant activity/ultrasound-assisted alkali extraction引用本文复制引用
基金项目
国家自然科学基金(31760470)
云南省兴滇英才支持计划青年人才专项(YNWR-QNBJ-2018-046)
出版年
2024
国家科技期刊平台
NSTL
万方数据