摘要
目的:研究三叶青提取物与其抗氧化活性的谱效关系,初步明确其发挥抗氧化活性的质量标志物.方法:制备三叶青提取物上大孔树脂柱,分别使用浓度为10％、30％、50％、70％、90％和100％的乙醇进行洗脱,得到6个不同洗脱部位;采用超高效液相色谱-四极杆-飞行时间串联质谱法(UPLC-Q-TOF-MS)建立三叶青提取物及各洗脱部位的指纹图谱并筛选特征峰;以1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率为抗氧化指标,考察三叶青提取物及各洗脱部位的抗氧化活性;再综合Pearson相关系数法和正交偏最小二乘(OPLS)法分析研究特征峰与抗氧化活性的谱效关系,筛选其质量标志物并分析鉴定其化学成分.结果:在正、负离子模式下,三叶青提取物及各洗脱部位分别筛选出57、92个特征峰;综合Pearson相关系数与OPLS分析结果,其中14个离子峰对抗氧化活性贡献较大,分别为香兰素(P2)、儿茶素(P13、N19)、芦丁(P27、N54)、异槲皮苷(P28)、山奈酚-3-O-芸香糖苷(P33、N61)、原花青素B(N18)、原花青素C(N22)以及离子峰N23、N25、N26、N29.结论:建立了三叶青提取物及各洗脱部位的UPLC-Q-TOF-MS指纹图谱,揭示了三叶青抗氧化活性的质量标志物,为三叶青的质量标准制定及资源开发利用提供技术支撑.
Abstract
Objective:To study the spectrum-effect relationship between the extracts of Tetrastigma hemsleyanum and its antioxidant activity,and to clarify the quality markers of its antioxidant activity.Methods:The extract was prepared and separated by macroporous resin elution,six different elution sites were obtained using ethanol at concentrations of 10％,30％,50％,70％,90％and 100％.The fingerprints of the Tetrastigma hemsleyanum extract and each elution site were established by UPLC-Q-TOF-MS and the characteristic peaks were screened.The free radical scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)was used as an antioxidant index to investigate the antioxidant activity of the extract and each elution site of Tetrastigma hemsleyanum.The Pearson correlation coefficient method and orthogonal partial least squares(OPLS)method were combined to analyze and study the spectrum-effect relationship between the characteristic peaks and the antioxidant activity,to screen the quality markers,and to analyze and identify the chemical compositions.Results:Under the positive and negative ion modes,57 and 92 characteristic peaks were identified in the extract and each elution site of Tetrastigma hemsleyanum,respectively.The combined results of Pearson correlation coefficient and OPLS analysis showed that 14 ion peaks contributed more to the antioxidant activity,which was vanillin(P2),catechin(P13,N19),rutin(P27,N54),and isoquercitrin(P28),kaempferol-3-O-rutinoside(P33,N61),proanthocyanidin B(N18),proanthocyanidin C(N22),and ion peaks N23,N25,N26,and N29,respectively.Conclusion:UPLC-Q-TOF-MS fingerprints of Tetrastigma hemsleyanum extract and each elution site were established,and the quality markers of Tetrastigma hemsleyanum antioxidant activity were revealed to provide technical support for the formulation of quality standards and resource development and utilization of Tetrastigma hemsleyanum.
基金项目
浙江省"领雁"研发攻关计划项目(2022C01174)
国家科技期刊平台
NSTL
万方数据