摘要
为促进优质天然富硒植物资源的科学开发利用,本文选取富硒金花葵为研究对象,采用氢化物发生-原子荧光光谱法(Hydride Generation-Atomic Fluorescence Spectrometry,HG-AFS)检测不同植物器官样品中的硒含量,超声波辅助乙醇提取金花葵花朵中的含硒有机活性组分并使用响应面法优化获得最佳提取工艺,同时对含硒提取物开展抗氧化活性研究.结果显示,金花葵各器官中硒含量从高到低依次为叶、果、花、根和茎,分别为21.99、14.29、12.93、10.90和10.23 mg/kg.响应面优化结果表明,含硒有机活性成分最佳提取参数为提取温度40℃、固液比1:60、提取时间40 min、超声功率350 W、乙醇浓度50％(V/v)和提取次数1次.在最佳提取参数下,硒的提取率为35.65％±0.65％.DPPH抗氧化活性测定结果显示,含硒有机活性成分的半抑制浓度(IC50)为0.048 mg/mL(硒元素浓度为0.79 μg/mL),羟基自由基清除实验显示样品浓度为8 mg/mL时达到最大清除率97％,1C50为0.56 mg/mL,还原力实验表明样品的还原能力随样品浓度的增大而增强.证明金花葵的含硒乙醇提取物具有良好的抗氧化能力.本研究可以为合理利用富硒金花葵和研发加工富硒农副作物提供一些理论依据.
Abstract
To promote scientific progress and optimize the utilization of natural plant resources that rich in selenium,the study selected selenium-rich Hibiscus manihot L.as the research object,using the HG-AFS to detect the selenium content in different plant organ samples,ultrasound assisted ethanol extraction of selenium containing organic active components from sunflower flowers,and obtaining the optimal extraction process by response surface methodology.At the same time,conducting antioxidant activity research on selenium containing extracts.The results showed that the total Se concentrations in different organs of Hibiscus manihot L.,from highest to lowest were found in the leaves,fruits,flowers,roots,and stems,with concentrations of 21.99,14.29,12.93,10.90,and 10.23 mg/kg,respectively.The RSM optimization results indicated that the ideal conditions for the extraction parameters were as follows:Extraction temperature of 40 ℃,solid-liquid ratio of 1:60,extraction time of 40 minutes,ultrasonic power of 350 W,ethanol concentration of 50％(VN),under one-time extraction.For the optimal conditions,the selenium extraction rate was 36.65％±0.65％.The DPPH antioxidant activity analysis showed a half-inhibitory concentration(IC50)value of 0.048 mg/mL(with a Se concentration of 0.79 μg/mL),the hydroxyl radical scavenging analysis showed that the maximum clearance rate was 97％when the extracts concentration was 8 mg/mL,and the IC50 was 0.56 mg/mL.The reducing power analysis showed that the reducing power of the extracts increases with the increase of the concentration,indicating that the extracts possess a good antioxidant capacity.The present study would provide empirical evidence to support the efficient use of selenium-rich Hibiscus manihot L.and the extraction of selenium-rich agricultural by-products.
基金项目
陕西省创新能力支撑计划-青年科技新星项目(2021SR5101)
陕西省安康市科学技术研究发展计划项目(AK2020-FX01-9)
维普
万方数据