超声波联合乙醇改性对猪心蛋白结构和功能的影响
Effect of Ultrasonic Combined with Ethanol Modification on the Structural and Functional Properties of Porcine Heart Protein
陆今明 1尚永彪 2侯大军 2蔡秋杏1
作者信息
- 1. 北部湾大学食品工程学院,广西钦州 535011;广西高校北部湾海产品高值化利用与预制食品重点实验室,广西钦州 535011
- 2. 西南大学食品科学学院,重庆 400715
- 折叠
摘要
为改善猪心蛋白的功能性质,提高其在食品工业中的应用可行性,本研究通过超声波(频率20 kHz,功率350 W,处理时间8 min,工作5s间歇2s)联合不同浓度乙醇(0～80％)处理对猪心蛋白(porcine heart protein,PHP)进行改性,测定改性后PHP的分子结构、微观结构、活性巯基含量、表面疏水性、溶解度、乳化性、乳液粒径及乳液Zeta电位值等指标的变化情况.结果表明,在超声波和40％浓度乙醇改性处理的共同作用下,PHP的一级结构、二级结构以及微观结构发生明显改变,PHP的紫外吸光强度最大,活性巯基含量及表面疏水性最大,蛋白的溶解性显著降低(P＜0.05),乳化活性及乳化稳定性改善效果最佳,蛋白乳液粒径最小、Zeta电位绝对值最大.综合考虑PHP乳化特性的改善效果,超声波联合40％浓度的乙醇改性处理为PHP的最佳改性条件.
Abstract
In order to improve the functional properties of porcine heart protein and improve its application feasibility in the food industry,this study modified porcine heart protein(PHP)by ultrasonic(frequency of 20 kHz,power of 350 W,treatment for 8 min,working 5 s with 2 s intervals)combined with different concentrations of ethanol(0～80％).The changes of molecular structure,microstructure,active sulfhydryl content,surface hydrophobicity,solubility,emulsification,emulsion particle size and emulsion Zeta potential of modified PHP were determined.The results showed that the primary structure,secondary structure and microstructure of PHP were significantly changed under the combined action of ultrasonic and 40％ethanol modification.The ultraviolet absorption intensity of PHP was the largest,the active sulfhydryl content and surface hydrophobicity were the largest.The solubility of protein was significantly reduced(P＜0.05),the emulsifying activity and emulsifying stability were the best,the particle size of protein emulsion was the smallest,and the absolute value of Zeta potential was the largest.Considering the improvement effect of PHP emulsification characteristics,ultrasonic combined with 40％ethanol modification treatment is the best modification condition for PHP.
关键词
超声波/乙醇/猪心蛋白改性/蛋白结构/蛋白功能特性Key words
ultrasonic/ethanol/porcine heart protein(PHP)modification/protein structure/protein functional properties引用本文复制引用
基金项目
重庆市技术创新与应用发展专项(cstc2020jscxtpyzxX0011)
出版年
2024
国家科技期刊平台
NSTL
万方数据