槐糖脂对玉米醇溶蛋白-槲皮素纳米颗粒结构和性质的影响
Effects of Sophorolipids on the Structure and Properties of Zein-Quercetin Nanoparticles
徐雪峰 1代德财 1闫浩 1杜金风1
作者信息
- 1. 海南科技职业大学化学与材料工程学院,海南海口 571126
- 折叠
摘要
本研究制备了玉米醇溶蛋白-槲皮素纳米颗粒(Zein-Q),采用扫描电子显微镜、傅里叶红外光谱和荧光光谱等方法分析了槐糖脂(SL)对Zein-Q颗粒结构的影响,并研究了 Zein-Q-SL 复合纳米颗粒的抗氧化活性和抑菌性质.结果表明,Zein-Q纳米颗粒为平均粒径128 nm的球形结构,包封率和载药量分别为25.8％±1.28％和3.98％±0.24％,对DPPH·和ABTS+·的清除率为76.8％±0.9％和65.4％±1.2％.加入槐糖脂有助于形成分散性较好的纳米颗粒,且槐糖脂在氢键、静电作用力等作用下包覆于玉米醇溶蛋白-槲皮素纳米颗粒外层,形成粒径较大的球形结构,当Zein-Q∶SL为3∶4时,Zein-Q-SL复合纳米颗粒的包封率和载药量分别增加至73.6％±1.80％和7.98％±0.41％,对DPPH·和ABTS+·的清除率分别达到88.8％±1.0％和78.6％±1.5％.此外,复合纳米颗粒对大肠杆菌和金黄色葡萄球菌显示出较好的抑制活性.由此表明,生物表面活性剂槐糖脂有潜力作为提高玉米醇溶蛋白-槲皮素纳米颗粒结构稳定性和活性的修饰分子.
Abstract
In this study,zein-quercetin(Zein-Q)nanoparticles were prepared and the effects of sophorolipids(SL)on the structure of Zein-Q nanoparticles were evaluated based on the combination of scanning electron microscopy,Fourier infrared spectroscopy and fluorescence spectroscopy.Their antioxidant and antibacterial properties were analyzed as well.The results showed that Zein-Q nanoparticles were spherical with an average particle size of 128 nm,the encapsulation efficiency and loading capacity were separately 25.8％±1.28％and 3.98％±0.24％,respectively,and its scavenging ability on DPPH and ABTS+.were 76.8％±0.9％and 65.4％±1.2％.The addition of sophorolipids resulted in forming nanoparticles with good dispersion.The structural characteration results showed that sophorolipids were coated with the outer layer of zein-quercetin nanoparticles driven by the interaction of hydrogen bonding and electrostatic interaction,forming a spherical core-cell structure with a larger particle size.At Zein-Q∶SL=3∶4,the encapsulation efficiency and loading capacity of composite Zein-Q-SL nanoparticles were increased to 73.6％±1.80％and 7.98％±0.41％,respectively.The antioxidant activity of zein-quercetin nanoparticles was enhanced,which showed that the scavenging ability of composite Zein-Q-SL nanoparticles on DPPH and ABTS+·reached 88.8％±1.0％and 78.6％±1.5％,respectively.In addition,the composite Zein-Q-SL nanoparticles showed a good inhibitory activity against Escherichia coli and Staphylococcus aureus.Thus,it can be concluded that biosurfactant sophorolipids have potential to be excellent modifier for enhancing the structural stability and activity of zein-quercetin nanoparticles.
关键词
槐糖脂/槲皮素/玉米醇溶蛋白纳米颗粒/抗氧化活性/抑菌活性Key words
sophorolipids/quercetin/zein nanoparticles/antioxidant activity/antibacterial activity引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据