基于Fe-HHTP@ZIF-8构建可视化传感器测定饮料的总抗氧化能力
Colorimetric Biosensor Based on Fe-HHTP@ZIF-8 for the Detection of Total Antioxidant Capacity in Beverages
武存慧 1秦之阳 1张海枝1
作者信息
- 1. 武汉轻工大学食品科学与工程学院,湖北武汉 430023
- 折叠
摘要
食品的总抗氧化能力是评价其功效的关键指标之一,建立快速灵敏的分析方法用于食品总抗氧化能力检测具有十分重要的意义.本文首次设计合成了一种具有类过氧化物酶活性的金属-有机框架复合物纳米酶(Fe-HHTP@ZIF-8),并对其形貌、结构及催化活性进行了详细表征.结果表明,Fe-HHTP@ZIF-8是一种具有二维超薄片层结构的纳米酶,计算得到Fe-HHTP@ZIF-8纳米酶对H2O2的Km和Vmax分别为0.67 mmol/L和3.55×10-8 mol/L·s-1.进一步以抗坏血酸(Ascorbic acid,AA)作为典型抗氧化剂,基于其对纳米酶催化显色反应的抑制作用,构建了一种高效的可视化传感器方法.系统验证表明,该可视化传感器检测限低(1.67μmol/L),线性范围宽(5.0～3000.0 μmol/L),准确度高(回收率为98.62％～103.13％).利用此传感器对不同饮料的总抗氧化能力进行检测,结果与样品标示值基本一致.因此,本研究为食品总抗氧化能力的评价提供了一种简单、快捷、有效的可视化新方法.
Abstract
Total antioxidant capacity of food is one of the critical parameters to evaluate its efficacy.Establishment of a fast and sensitive analytical method for the detection of total antioxidant capacity of food has great significance.In this paper,a metal-organic framework complex nanozyme(Fe-HHTP@ZIF-8)with peroxidase-like activity was firstly designed and successfully synthesized.Then,the morphology,structure and catalytic activity were characterized in detail.The results showed that Fe-HHTP@ZIF-8 possessed a two-dimensional ultra-thin layer structure while its Km and Vmax values towards H2O2 were 0.67 mmol/L and 3.55×10-8 mol/L·s-1,respectively.Ascorbic acid(AA)as a typical antioxidant,an efficient colorimetric biosensor was constructed based on its inhibitory effect on chromogenic reaction catalyzed by nanozyme.Comprehensive validation showed that the colorimetric biosensor had low limit of detection(1.67 μmol/L),wide linear range(5.0～3000.0 μmol/L)and high accuracy(recovery rate of 98.62％～103.13％).By using this colorimetric method,total antioxidant capacity of different beverages was detected and the obtained results were basically consistent with labeled values.Therefore,this study provided a simple,fast and effective colorimetric method for the evaluation of the total antioxidant capacity of food.
关键词
总抗氧化能力/比色检测/过氧化物模拟酶Key words
total antioxidant capacity/colorimetric detection/peroxidase-like nanozyme引用本文复制引用
基金项目
国家自然科学基金项目(32302220)
湖北省教育厅项目(T2022023)
出版年
2024
国家科技期刊平台
NSTL
万方数据