In order to improve the hydrophobicity and volatility of terpinen-4-ol and extend its duration of action,β-cyclodextrin-based metal-organic framework(β-CD-MOF)was first prepared by the modified vapor diffusion method,and then terpinen-4-ol was encapsulated with the β-CD-MOF to prepare a terpinen-4-ol/β-CD-MOF inclusion complex by the solvent incubation method.The mass ratio of the prepared β-CD-MOF or β-CD used as the control to terpinen-4-ol was optimized for encapsulation.The morphological characteristics,spectral absorption properties and thermogravimetric characterisation of the terpinen-4-ol/β-CD-MOF complex were analyzed.Through in vitro bacteriostatic assays,the inhibitory effects of the complex on three food-borne pathogens were determined.The results showed that the highest drug loading and encapsulation efficiency were achieved when the mass ratio of terpinen-4-ol to β-CD-MOF was 1∶1,superior to the same preparation conditions using β-CD as a carrier.Scanning electron microscope(SEM)observations indicated that irregularly arranged β-CD molecules stacked together under the bridging action of K+,forming an orderly arranged rectangular β-CD-MOF.Fourier-transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)analysis demonstrated terpinen-4-ol was successfully encapsulated in β-CD-MOF with hydrogen bond interactions between them.Thermogravimetric analysis(TGA)proved that the volatilization rate of terpinen-4-ol could be retarded when encapsulated by β-CD-MOF,extending its application temperature range from 30～160 ℃ to 30～350 ℃.In vitro antibacterial experiments showed that the terpinen-4-ol/β-CD-MOF complex exhibited long-lasting antibacterial abilities against three common cold-chain microorganisms,including Escherichia coli,Staphylococcus aureus and Listeria monocytogenes.In conclusion,encapsulating terpinen-4-ol in β-CD-MOF allows for effective preservation,providing a theoretical basis for the development of novel,green and safe antimicrobial materials for cold-chain applications.
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