Objective:To investigate the protective effect and mechanism of abalone visceral polysaccharide-protein complex selenium nanoparticles(Abalone Visceral Polysaccharide-protein Complex Selenium Nanoparticles,PSP-SeNPs)on alcohol-induced damage in AML12 cells.Methods:After pretreated with different levels of PSP-SeNPs(0.1,0.2,0.3,and 0.4 µg/mL)for 24 h,AML12 cells were further treated with 300 mmol/L alcohol for 24 h to establish cell injury model.Hoechst staining was used to assess the degree of cell injury,and biochemical indicators related to oxidative stress were measured.Finally,qRT-PCR was used to measure the expression of oxidative stress-related genes in AML-12 cells.Results:Compared with the normal control group,the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and catalase(CAT)in the alcohol model group were significantly decreased(P＜0.05),while the levels of malondialdehyde(MDA)and reactive oxygen species(ROS)were significantly increased(P＜0.05).These changes were significantly reversed by PSP-SeNPs(P＜0.05).qRT-PCR results showed that the mRNA levels of nuclear factor E2-related factor 2(Nrf2),superoxide dismutase 2(SOD2),catalase(CAT),and glutamate-cysteine ligase regulatory subunit(GCLM)were significantly decreased in the alcohol model group as compared with the control group(P＜0.05).Additionally,the alcohol induced up-regulation of Kelch-like ECH-associated protein 1(Keap1)mRNA was significantly reversed by PSP-SeNPs(P＜0.05).Conclusion:PSP-SeNPs can alleviate alcohol-induced AML 12 cell injury by regulating the expression levels of oxidative stress-related genes and modulating the activities of antioxidant enzymes.
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