Objective:To isolate and purify the polysaccharides from Lycium barbarum and study its in vitro antioxidant activity and in vivo anti-aging effect.Methods:Crude polysaccharides from Lycium barbarum were obtained by water extraction and alcohol precipitation,and then LBP was isolated and purified from crude polysaccharides by Sevage reagent and DEAE-52 cellulose ion exchange resin.By measuring the scavenging ability of LBP to DPPH free radical,hydroxyl radical and ABTS+free radical,and Fe3+reduction,the antioxidant ability of LBP in vitro was evaluated.The aging mouse model was established by D-galactose.After administration,the body weights and organ indexes of mice in each group were compared.The levels of MDA,SOD,GSH-Px and CAT in serum,liver and brain were measured.The protein expression of Nrf-2 and HO-1 in liver tissue was detected by Western blotting.Results:The content of LBP after isolation and purification was 86.64％±2.34％.The IC50 values of scavenging ability of LBP to DPPH free radical,hydroxyl radical and ABTS+free radical were 0.2081±0.0182,0.7132±0.0220 and 0.3646±0.0138 mg/mL,respectively.Compared with the model group,the body weights and organ indexes of the mice in positive and high dose of LBP groups were significantly increased(P＜0.05 or P＜0.01).The levels of SOD,CAT and GSH-Px in serum,liver and brain of mice in positive and high dose of LBP groups were significantly increased,and the level of MDA was significantly decreased(P＜0.01).The expression level of Nrf-2 protein in liver tissue increased significantly except for in the low dose of LBP group(P＜0.05),and the expression level of HO-1 protein increased significantly in all groups(P＜0.05 or P＜0.01).Conclusion:LBP from Lycium barbarum has strong antioxidant ability and certain anti-aging effect,and its mechanism may be related to Nrf-2/HO-1 signal pathway.
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