摘要
为深入研究铁皮石斛多糖的抗氧化活性,以铁皮石斛为原料,提取纯化铁皮石斛多糖并表征其初步结构,从铁皮石斛多糖自由基清除能力和对低密度脂蛋白(Low Density Lipoprotein,LDL)体外氧化抑制效果两个方面评价其抗氧化活性.结果表明,铁皮石斛多糖总糖含量90.17％±3.90％,蛋白质含量0.14％±0.05％,灰分含量4.47％±1.42％以及糖醛酸含量2.53％±1.02％,相对分子量23970 Da,单糖组成主要为甘露糖和葡萄糖.铁皮石斛多糖具有一定的自由基清除能力,可以抑制LDL氧化修饰体系中共轭二烯的产生,3.00mg·mL-1时铁皮石斛多糖对铜离子诱导的LDL氧化产生的硫代巴比妥酸反应产物(Thiobarbituric Acid Reactive Substances,TBARS)抑制率为57.46％±0.56％.本研究可为开发因LDL氧化导致的动脉粥样硬化(Arteriosclerosis,AS)等疾病药物提供理论支撑.
Abstract
To study the antioxidant activity of polysaccharides from Dendrobium officinale,the polysaccharide was extracted and purified from Dendrobium officinale,and its preliminary structure was characterized.Free radical scavenging activity and inhibition of low density lipoprotein(LDL)in vitro oxidation of Dendrobium officinale polysaccharides were evaluated.The results showed that the total sugar content of the obtained Dendrobium officinale polysaccharide was 90.17％±3.90％,the protein content was 0.14％±0.05％,the ash content was 4.47％±1.42％and the glucuronic acid content was 2.53％±1.02％,the relative molecular weight was 23970 Da and the monosaccharides were mainly mannose and glucose in this test.Dendrobium officinale polysaccharide had certain free radical scavenging capacity.It could inhibit the production of conjugated diene in the oxidative modification system of LDL.The inhibition rate of thiobarbituric acid reactive substances(TBARS)by copper ion reached 57.46％±0.56％when the polysaccharide concentration was 3.00 mg·mL-1.This study could provide theoretical support for the development of drugs for diseases such as arteriosclerosis due to LDL oxidation.
维普
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