This paper aimed to investigate the antioxidant activity of buckwheat bee pollen polysaccharides(BWPP)during the in vitro gastrointestinal digestion.The crude polysaccharides were purified using DEAE-52 cellulose column and their antioxidant activity during in vitro simulated gastric and intestinal digestion was studied.The results showed that after purification by DEAE-52 cellulose column chromatography,the highest total sugar content component BWPP-1 was obtained,which was mainly composed of Glc,Xyl,Ara,etc.The infrared spectrum showed that BWPP-1 had obvious polysaccharide characteristic absorption peaks.During the simulated in vitro gastrointestinal digestion,the ABTS+·scavenging rates of BWPP and BWPP-1 reached their maximum values at 60 minutes of simulated gastric juice digestion,which were 86.39％±1.28％and 90.38％±2.78％,respectively.There was a significant difference compared to other digestion times(P＜0.05).The DPPH clearance rates reached their maximum values at 60 minutes,which were 86.86％±0.11％and 89.58％±1.67％,respectively,and the difference between the two was not significant(P＞0.05).When simulating intestinal digestion,the maximum clearance rates of ABTS+.by BWPP and BWPP-1 reached 88.67％±0.40％and 91.82％±2.77％at 60 minutes,and the difference between the two was not significant(P＞0.05).The DPPH scavenging rates reached their peak values at 240 minutes of 57.11％±0.06％and 65.67％±3.67％,with significant difference(P＜0.05).During the digestion process,the ability to reduce iron ions was relatively weak.Based on these findings,it can be concluded that BWPP and BWPP-1 have good ABTS+.and DPPH scavenging abilities,and the DPPH·scavenging ability during gastric digestion is slightly higher than that during intestinal digestion,while the ABTS+.scavenging ability during intestinal digestion is slightly higher than that during gastric digestion.This study provides a theoretical basis for the development of functional products based on buckwheat bee pollen.
维普
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