摘要
为分析山东临沂地区高温大曲细菌类群及其理化特性,本研究采用Illumina MiSeq高通量测序技术和纯培养技术对兰陵酒业高温大曲中细菌类群进行了解析,通过常规检测方法对其理化指标进行了测定,探究大曲中细菌类群与理化指标之间的关联性.结果表明,大曲中细菌属主要为克罗彭斯特德菌属(Kroppenstedtia)、高温放线菌属(Thermoactinomyces)、糖多孢菌属(Saccharopolyspora)、葡萄球菌属(Staphylococcus)、红球菌属(Rhodococcus)、魏斯氏菌属(Weissella)和芽孢杆菌属(Bacillus)等.Bacillus subtilis、Bacillus licheniformis和Bacillus coagulans为可培养优势细菌分离株.15份高温大曲间糖化力、酯化力、酒化力和液化力差异较大.Kroppenstedtia、Staphylococcus、Leucobacter、Rhodococcus 和 Weissella 与酯化力、发酵力、酒化力、酯化力和液化力间呈现显著相关性(P＜0.05).由此可见,兰陵酒业高温大曲中存在大量核心细菌类群,且各细菌属与理化指标间存在明显相关性,进一步为该地区高温大曲品质的改善与提升提供参考.
Abstract
In order to analyze the high-temperature Daqu bacterial taxa and their physicochemical properties in Linyi area,Shandong Province,Illumina MiSeq high-throughput sequencing technology and pure culture technology were used to analyze the bacterial taxa of high-temperature Daqu in Lanling wine,and then the physicochemical indexes were determined by conventional detection methods in this study.Finally,the correlation between bacterial taxa and physicochemical indexes in Daqu was explored.The results showed that the main genera of bacteria in Daqu were Kroppenstedtia,Thermoactinomyces,Saccharopolyspora,Staphylococcus,Rhodococcus,Weissella and Bacillus,et al.Bacillus subtilis,Bacillus licheniformis and Bacillus coagulans were cultureable dominant bacterial isolates.There was a large difference in saccharification,esterification,alcoholization and liquefaction between the 15 parts of high-temperature Daqu.Kroppenstedtia,Staphylococcus,Leucobacter,Rhodococcus and Weissella were significantly positively correlated with esterification,fermentation,alcoholization,esterification and liquefaction(P＜0.05).Thus,there are a large number of core bacterial taxa in high-temperature Daqu in Lanling wine,and there is a significant correlation between various bacterial genera and physicochemical indexes,which further provides a reference for the improvement and enhancement of the quality of high-temperature Daqu in this area.
维普
万方数据