Objective:To explore the feasibility of using stable carbon and oxygen isotopes and mineral elements in small-scale regional wine production identification,the fractionation characteristics of stable isotopes of carbon and oxygen in wine of Matheran single wine from different sub-producing areas at the eastern foot of Helan Mountain in Ningxia were studied.Methods:Forty-five samples of Massellan single wine from 5 sub-producing areas of Hongsipu,Qingtongxia,Yongning,Helan,and Zhenbeipu were selected to analyze the mineral element contents and the values of δ13C and δ18O.The fisher linear discriminant analysis method was used to establish a wine region discriminant model based on stable isotopes and mineral elements.Result:Carbon and oxygen stable isotopes exhibited significant fractionation during wine fermentation,with a total δ13C>ethanol δ13C>glycerol δ13C,and the three showed a certain degree of homology.The order of the δ18O size was grape juice δ18O>wine δ18O>water δ18O.As、B、K、Li、Mn、Ni、Pb、Rb、Sb、Sr、Ti and Cs showed significant differences between some production areas(P<0.05).There was no significant difference in the distribution ofδ13C,δ18O,Ba,Ca and other 13 mineral elements among different regions(P>0.05).The accuracy rate of origin discrimination based on stable carbon and oxygen isotopes was only 40%,while the accuracy rates based on mineral elements and carbon and oxygen stable isotopes combined with mineral elements were both 95.6%.Conclusion:Mineral elements can distinguish wine samples from different sub-producing areas effectively.Stable carbon and oxygen isotopes cannot be used to identify the producing areas of wine from different small-scale regions.
关键词
贺兰山东麓葡萄酒/子产区/稳定同位素/矿物元素/产地识别
Key words
wine at the eastern of Helan Mountain/sub-producing area/stable isotope/mineral elements/origin identification