To improve the utilization of by-products of the Hyriopsis cumingii,this study investigated the effect of Hyriopsis cumingii polysaccharide(HCP)on the proliferation and migration of normal cells,as well as its reparative effects on ultraviolet radiation-B(UVB)-damaged cells.First,HCP was isolated and purified using DEAE Cellulose-52 column chromatography,Sephadex G-100 column chromatography and nanofiltration.Then,structural analysis was conducted using high-performance liquid chromatography,high-performance gel permeation chromatography,and infrared spectroscopy.Following this,cell proliferation experiments,cell scratch tests,UVB damage experiments,and protein expression analyses were employed to explore the anti-UVB damage effects and corresponding mechanisms of polysaccharide components.The results indicated the three substances obtained by isolation and purification were all homogeneous polysaccharide components.Among them,HCP-2 played a major role in promoting cell proliferation in HCP and facilitated the migration of HaCaT cells.The recovery of UVB-damaged cell vitality was raised by the increasing concentration of HCP-2.Furthermore,the reparative effect was more substantial than the protective effect at the same sample concentration.It was also observed that HCP-2 up-regulated the expression of the anti-apoptotic protein bcl-2 while inhibited the expression of apoptosis-related proteins casp9,p-Akt,and p-p38,indicating that HCP-2 could repair HaCaT cells by regulating apoptosis of cells damaged by UVB.In conclusion,HCP had the ability to inhibit cell apoptosis which provide a theoretical basis for its future application in the development of food and health products.
维普
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