摘要
目的:探究大鲵蛋白肽(CGS)及其美拉德反应产物(CGSXyU)对Ⅱ型糖尿病小鼠降血糖血脂、免疫低下小鼠免疫调节功能及正常小鼠抗疲劳功能活性的影响.方法:将小鼠随机分为空白对照组、模型对照组、阳性对照组(200 mg/kg盐酸二甲酸胍)、低剂量样品组(CGS/CGSXyU-L,50 mg/kg)和高剂量样品组(CGS/CGSXyU-H,200 mg/kg),使用100 mg/kg四氧嘧啶建模成功后,灌胃3周,测定小鼠空腹血糖浓度、血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;将小鼠随机分为空白对照组、模型对照组、阳性对照组(50mg/kg左旋咪唑)、低剂量样品组(CGS/CGSXyU-L,50 mg/kg)和高剂量样品组(CGS/CGSXyU-H,200 mg/kg),使用 100 mg/kg 环磷酰胺建模成功后,灌胃3周,记录小鼠体重并测定免疫低下小鼠脏器指数变化以及细胞因子含量变化;将小鼠随机分为空白对照、阳性对照组(200 mg/kg牛磺酸)、低剂量样品组(CGS/CGSXyU-L,50 mg/kg)和高剂量样品组(CGS/CGSXyU-H,200 mg/kg),灌胃4周,测定小鼠负重游泳时间、尿素氮及肌酸激酶、血清乳酸含量及抗氧化酶活性.结果:CGS/CGSXyU-L/H处理组小鼠空腹血糖浓度、血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)均有不同程度的降低,高密度脂蛋白胆固醇(HDL-C)水平提高;CGSXyU-H可以显著(P＜0.05)促进免疫低下小鼠体重、脏器指数升高以及免疫细胞因子(IL-6、IL-10、TNF-α、IFN-γ )含量升高;CGS/CGSXyU-L/H处理组负重游泳时间升高,肌酸激酶活性、血清乳酸含量降低,CGS-H以及CGSXyU-L/H处理组尿素氮含量下降,CGSXyU-H处理组GSH-Px活性显著上升(P＜0.05),CGS-H以及CGSXyU-L/H处理组SOD活性上升.结论:大鲵蛋白肽及其美拉德反应产物可调节Ⅱ型糖尿病小鼠血糖及血脂代谢,增强免疫低下小鼠免疫功能并有效提升正常小鼠的抗疲劳能力.
Abstract
Objective:To investigate the impact of giant salamander protein peptide(CGS)and its Maillard reaction product(CGSXyU)on lowering blood glucose and lipid levels in type Ⅱ diabetic mice and enhancing immunomodulatory functions in immunosuppressed mice,and anti-fatigue effects in normal mice.Methods:Mice were randomly assigned to a blank control group,a model control group,a positive control group(200 mg/kg metformin),a low-dose sample group(CGS/CGSXyU-L,50 mg/kg),and a high-dose sample group(CGS/CGSXyU-H,200 mg/kg).Following the successful induction of diabetes with 100 mg/kg alloxan,the mice underwent gavage treatment for 3 weeks,after which their fasting blood glucose levels,serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)levels were measured.Mice were randomly assigned to a blank control group,a model control group,a positive control group(50 mg/kg levamisole),a low-dose sample group(CGS/CGSXyU-L,50 mg/kg),and a high-dose sample group(CGS/CGSXyU-H,200 mg/kg).Following successful modeling with 100 mg/kg cyclophosphamide,the mice underwent gavage for three weeks,during which their body weights were recorded,and changes in organ indices and cytokine levels were assessed.Similarly,mice were randomly divided into a blank control group,a positive control group(200 mg/kg taurine),a low-dose sample group(CGS/CGSXyU-L,50 mg/kg),and a high-dose sample group(CGS/CGSXyU-H,200 mg/kg).They were subjected to gavage for four weeks,after which the mice's weight-bearing swimming time,urea nitrogen levels,creatine kinase activity,serum lactate levels,and antioxidant enzyme activity were measured.Results:The CGS/CGSXyU-L/H treated group of mice exhibited reduced fasting blood glucose concentration,serum total cholesterol(TC),triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C),along with elevated high-density lipoprotein cholesterol(HDL-C)levels.CGSXyU-H significantly enhanced the body weight(P＜0.05),organ index,and levels of immune cytokines(IL-6,IL-10,TNF-α,IFN-γ)in immunocompromised mice.The CGS/CGSXyU-L/H treatment group demonstrated increased time in weight-bearing swimming,reduced creatine kinase activity and serum lactate levels,the BUN level in the CGS-H and CGSXyU-L/H groups decreased,while the GSH-Px activity significantly increased in the CGSXyU-H group(P＜0.05),the SOD activity in the CGS-H and CGSXyU-L/H groups also increased.Conclusion:The protein peptides from Andrias davidianus and their Maillard reaction products regulate blood glucose and lipid metabolism in type Ⅱ diabetes mice,bolster immune function in immunocompromised mice,and effectively improve the anti-fatigue capacity in normal mice.
维普
万方数据