响应面法优化山楂多酚微粒制备工艺
Optimization of Preparation Process for Hawthorn Polyphenol Microparticles Based on Response Surface Methodology
井勇慧 1李桥 2康丽 1任飞燕 1王东微 3魏力军 2徐逸尔 2孙贵才 4王强 3刁岩3
作者信息
- 1. 西华师范大学生命科学学院,四川南充 637009
- 2. 哈尔滨工业大学生命科学与技术学院,黑龙江哈尔滨 150001
- 3. 西华师范大学生命科学学院,四川南充 637009;西华师范大学组织修复材料工程技术中心,四川南充 637009
- 4. 南昌大学第一附属医院,江西南昌 330006
- 折叠
摘要
本研究依据聚电解质自组装原理将山楂多酚制备为山楂多酚微粒(Hawthorn polyphenol microparticles,HPM).通过Plackett-Burman联用Box-Behnken设计,以山楂多酚的包封率和载药量为响应值对HPM的制备工艺因素进行研究.采用Plackett-Burman设计筛选对实验结果影响较为显著的因素(制备时间、硫酸浓度和包封材料比例)进行Box-Behnken响应曲面优化试验.利用SEM和TEM观测HPM的形貌,并利用模拟胃肠液研究HPM的胃肠道释放动力学.结果表明:制备时间、硫酸浓度和包封材料比例对HPM的制备具有明显影响,制备时间为3 h,硫酸浓度为0.0254％,壳聚糖与海藻酸钠比例为4:1时HPM的包封率为96.27％±1.37％.对HPM的形貌研究显示,其为不规则的圆形颗粒且分散性良好.HPM在模拟肠环境中的释放速度大于模拟胃环境.其在胃液和肠液中的释放分别属于零级和一级动力学.综上所述,本研究制备出一种包封率、载药量较高且分散性良好的HPM,并且制备的HPM可以降低胃肠道对山楂多酚的降解,为山楂多酚的食品添加提供了新的思路.
Abstract
This study synthesized hawthorn polyphenol microparticles(HPM)based on the polyelectrolyte self-assembly technology.Plackett-burman combined with Box-Behnken design was used to study the factors affecting the preparation of HPM in response to the encapsulation efficiency and drug loading of hawthorn polyphenols.The Plackett-Burman design was used to screen for factors that significantly affect the outcomes,such as preparation time,sulfuric acid concentration,and the ratio of encapsulating materials,which were optimized through Box-Behnken response surface methodology.The morphology of HPM was examined using scanning electron microscopy(SEM)and transmission electron microscopy(TEM),and their gastrointestinal release kinetics were assessed using simulated gastric and intestinal fluids.The results indicated that the preparation time,sulfuric acid concentration,and the ratio of encapsulating materials significantly effect the fabrication of HPM.An encapsulation efficiency of 96.27％±1.37％was achieved with a preparation time of 3 h,a sulfuric acid concentration of 0.0254％,and a 4:1 ratio of chitosan to sodium alginate.Morphological analysis of HPM revealed irregularly shaped,well-dispersed spherical particles.The release rate of HPM in simulated intestinal fluid was higher than in gastric fluid,with the release kinetics corresponding to zero-order in gastric fluid and first-order in intestinal fluid.In summary,this study successfully produced HPM with a high encapsulation efficiency,substantial drug loading,and good dispersion.The HPM formulated could reduce the degradation of hawthorn polyphenols in the gastrointestinal tract,offering a novel approach for incorporating hawthorn polyphenols into food products.
关键词
山楂多酚/自组装/壳聚糖/海藻酸钠/工艺优化/胃肠道释放/动力学Key words
hawthorn polyphenols/self-assembly/chitosan/sodium alginate/process optimization/gastrointestinal release/kinetics引用本文复制引用
基金项目
西华师范大学基础研究基金(20E058)
国家自然科学基金地区基金项目(82260870)
国家自然科学基金地区基金项目(81960881)
江西省重点研发计划"揭榜挂帅"项目(20223BBG71S01)
出版年
2024
国家科技期刊平台
NSTL
万方数据