赶黄草叶不同溶剂提取物中活性成分、抗氧化活性及胰脂肪酶抑制活性对比
Comparison of Active Components,Antioxidant Activity and Pancreatic Lipase Inhibitory Activity in Different Solvent Extracts of Penthorum chinense Leaves
刘佳 1向卓亚 2朱柏雨 2夏陈 2朱永清 1杨星 2陈松3
作者信息
- 1. 成都大学食品与生物工程学院,四川成都 610106;四川省农业科学院农产品加工所(四川省农业科学院食物与营养健康研究所),四川成都 610066
- 2. 四川省农业科学院农产品加工所(四川省农业科学院食物与营养健康研究所),四川成都 610066
- 3. 古萃(古蔺)生物科技有限公司,四川泸州 646500
- 折叠
摘要
赶黄草叶活性成分丰富,本文对赶黄草叶不同溶剂提取(80％丙酮、80％乙醇、80％甲醇、水)物中活性成分含量、体外抗氧化能力以及胰脂肪酶抑制能力进行对比研究.结果显示,80％丙酮提取物中总多酚含量最高(250.04 mg/g),而80％甲醇和80％乙醇提取物中总黄酮(176.96 mg/g和174.89 mg/g)和总原花青素含量(43.05 mg/g和42.87 mg/g)最高.除芦丁、异槲皮苷、山奈酚-3-O-芸香糖苷、乔松素-7-O-葡萄糖苷、乔松素-7-O-(3"-O-没食子酰基-4",6"-六羟基联苯二甲酰基)-β-葡萄糖苷和赶黄草苷A外,三种有机溶剂提取物中9种多酚类化合物含量无显著性差异,均显著高于水提物(P＜0.05).80％甲醇提取物和80％乙醇提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基(IC50=42.17 μg/mL和42.21 μg/mL,P＞0.05)清除能力最强,80％丙酮提取物对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)自由基清除能力最强(IC50=48.92 μg/mL),铁离子还原能力最强为80％乙醇提取物.此外,80％丙酮对体外胰脂肪酶抑制能力最强(IC50=0.46 mg/mL).相关性分析表明,赶黄草叶体外DPPH和ABTS+自由基清除能力以及胰脂肪酶抑制能力与11种多酚类化合物呈显著或极显著正相关(P＜0.05,P＜0.01).综上,结合不同溶剂提取物中活性成分含量、体外活性以及溶剂毒性影响,本研究建议将80％乙醇作为赶黄草叶提取溶剂,为赶黄草叶进一步开发提取利用提供理论依据.
Abstract
The Penthorum chinense Pursh leaves were rich in active ingredients.The active ingredient content,in vitro antioxidant capacity and pancreatic lipase inhibition ability of P.chinense leaves extracted by different solvents(80％acetone,80％ethanol,80％methanol,water)were compared in this study.The results showed that the total phenolic content(250.04 mg/g)extracted by 80％acetone was highest,while both the total flavonoid content(176.96 mg/g and 174.89 mg/g)and total proanthocyanidins content(43.05 mg/g and 42.87 mg/g)extracted by 80％methanol and 80％ethanol were highest.Except for rutin,isoquercitrin,kaempferol-3-O-rutinoside,pinocembrin 7-O-D-glucoside,pinocembrin 7-O-(3"-O-galloy-4",6"-hexahydroxydiphenoyl)-β-glucoside and thonningianin A,the content of nine phenolic compounds showed insignificant differences among three organic solvent extracts,which were significantly higher than those in water extract(P＜0.05).Both 80％ethanol extract and 80％methanol extract showed the strongest DPPH free radical scavenging ability(IC50=42.17 μg/mL and 42.21 μg/mL,P＞0.05),while 80％acetone extract showed the strongest ABTS+free radical scavenging ability(IC50=48.92 μg/mL)and 80％ethanol extract also showed the strongest ferric reducing antioxidant power.In addition,in vitro pancreatic lipase inhibitory activity of 80％acetone extract(IC50=0.46 mg/mL)was the strongest.Correlation analysis showed that DPPH and ABTS+free radical scavenging ability and pancreatic lipase inhibition ability were significantly or extremely significantly positively correlated with 11 phenolic compounds(P＜0.05,P＜0.0l).Above all,in view of the active ingredient content,in vitro biological activity and solvent toxicity,this study recommend 80％ethanol as the extract solvent of P.chinense leaves,which would provide the theoretical basis for the further development,extraction and utilization of P.chinense leaves.
关键词
赶黄草叶/溶剂提取/活性成分/抗氧化活性/胰脂肪酶抑制活性Key words
P.chinense leaves/solvent extract/functional ingredient/antioxidant activity/pancreatic lipase inhibitory activity引用本文复制引用
基金项目
四川省农业科学院"1+9揭榜挂帅"项目(1+9KJGG007)
四川道地中药材创新团队项目(SCCXTD-2020-19)
出版年
2024
国家科技期刊平台
NSTL
万方数据