利用双蛋白天然共生颗粒制备Pickering纳米乳液并对其物化稳定性进行分析,以此拓宽其在食品领域中的应用潜力.本研究采用碱提取-等电点沉淀法(Alkali extraction-isoelectric point precipitation,AE)和盐提取-透析法(Salt extraction-dialysis,SE)从大豆-亚麻籽混合粕中富集大豆蛋白(Soy protein)和亚麻籽蛋白(Flaxseed protein),制备双蛋白天然共生颗粒(Natural particles,SFNPs).首先,利用外观观察、原子力显微镜(Atomic force microscopy,AFM)、圆二色谱和拉曼光谱对两种双蛋白共生颗粒进行表征;其次,将其作为颗粒稳定剂制备Pickering纳米乳液,并对其物理和化学稳定性进行表征.结果表明AE-SFNPs的尺度更为均一,且具有良好的分散性.与SE-SFNPs相比,AE-SFNPs的二级结构中β-折叠占比更高,达51.25％,具有更加致密有序和良好柔性的结构特性.2种双蛋白天然共生颗粒均可制备出Pickering纳米乳液.在28 d的贮藏期间,AE-SFNPs稳定乳液的粒径仅增加了约14 nm,电位基本稳定在-34 mV左右,而SE-SFNPs稳定乳液的粒径增加了约12倍,电位降低了 1.32 倍.贮藏至第 28 d 时,AE-SFNPs 的稳定性指数(Turbiscan stability index,TSI,5.83),远小于 SE-SFNPs的TSI值(55.13).尤其是,AE-SFNPs制备的Pickering纳米乳液表现出了比酪蛋白酸钠(食品领域加工常用乳化剂)制备的纳米乳液更佳的物理稳定性和化学稳定性.因此,AE-SFNPs可作为一种天然高效植物蛋白基颗粒稳定剂制备Pickering纳米乳液,为丰富纳米乳液的工业化应用提供了新的解决策略.
Abstract
Pickering nanoemulsions were prepared using dual-protein natural symbiotic particles and their physicochemical stability was analyzed to broaden their potential application in the food industry.This study employed alkali extraction-isoelectric point precipitation(AE)and salt extraction-dialysis(SE)to enrich soy protein and flaxseed protein from soybean-flaxseed mixed meal for fabricating dual-protein symbiotic natural particles(SFNPs).First,two dual-protein symbiotic particles were characterized using appearance observation,AFM,circular dichroism,and Raman spectroscopy.Then,Pickering nanoemulsions were prepared with them as particle stabilizers and characterized for their physical and chemical stability.Results demonstrated that AE-SFNPs had a higher uniformity of size and better dispersibility.AE-SFNPs also showed a higher proportion(as high as 51.25％)of β-sheet in its secondary structure,endowing it a more tightly ordered and flexible structure compared with SE-SFNPs.Moreover,both AE-SFNPs and SE-SFNPs could be used to generate Pickering nanoemulsion.During 28 days of storage,the particle size of the stabilized emulsion of AE-SFNPs increased only about 14 nm and its ζ-potential was stabilized at about-34 mV.In contrast,the particle size of the stabilized emulsion of SE-SFNPs increased about 12 fold,and its ζ-potential was decreased 1.32 fold.The stability index(TSI,5.83)of the AE-SFNPs was much smaller than the TSI value(55.13)of the SE-SFNPs when stored until the 28th day.Notably,the Pickering nanoemulsion stabilized by AE-SFNPs exhibited higher physical and chemical stability than that prepared by sodium caseinate(a common emulsifier used in food processing).Therefore,AE-SFNPs can serve as a natural and efficient plant protein-based particle stabilizer to prepare Pickering nanoemulsion.This work provides a new strategy to enhance the industrial application of nanoemulsions.
关键词
植物基双蛋白/共生提取/天然颗粒/界面重构/Pickering纳米乳液
Key words
plant based dual-protein/symbiotic extraction/natural particles/interfacial remodeling/Pickering nanoemulsion
维普
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