Tartary buckwheat was used as raw material to extract tartary buckwheat protein,and the enzymatic hydrolysis conditions of tartary buckwheat protein lipid-lowering peptides were screened by single factor and response surface optimization experiments using the inhibition rate of pancreatic lipase as index.The amino acid composition and lipid lowering activity of lipid-lowering peptides were studied.The results showed that the lipid-lowering peptides obtained from tartary buckwheat protein with bromelain had higher inhibitory activity on pancreatic lipase.The optimal enzymatic hydrolysis conditions were hydrolysis time 2.1 h,hydrolysis temperature 62.0 ℃,substrate concentration 3.23％,pH7.0 and enzyme substrate ratio 0.4％.Under these conditions,the inhibitory rate of pancreatic lipase was 81.22％±0.16％.Amino acid analysis showed that essential amino acids,hydrophobic amino acids and acidic amino acids accounted for 27.82％,32.11％,35.86％,and the ratio of arginine to lysine was 2.28.The EC50/IC50 values of sodium taurine cholic acid binding rate,cholesterol esterase inhibition rate and pancreatic lipase inhibition rate were 9.136,3.902 and 0.051 mg/mL,respectively.The results provided a new idea and theoretical basis for the high-value utilization of tartary buckwheat resources and the development of tartary buckwheat protein active peptides.
维普
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