This work aimed to develop a high-throughput analytical method based on QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)for the determination of 13 mycotoxins in plant-solid beverages.The samples were homogenized and dissolved in water.QuEChERS method was used for pretreatment,ultrasonic extraction with 2％formic acid-acetonitrile solution,and purification with octadecylsilane bonded silica gel(C18).The extraction solution was then separated by a Kinetex F5 column,detected by a tandem mass spectrometry detector in multiple reaction monitoring mode(MRM),and quantified by matrix-matched standard calibration.The optimum conditions achieved limits of detections(LODs)in the range of 0.04～16.9 μg/kg,with correlation coefficient over 0.9916.The limit of quantifications(LOQs)were in the range 0.12～38.2 μg/kg.The recoveries in real plant-solid beverages ranged from 82.2％to 103.3％,with relative standard deviations(RSDs)below 9.0％.Moreover,the proposed method was applied to analyze mycotoxins in 18 plant-solid beverage samples,a positive detection rate of 4/18 was obtained,and the detected analytes included deoxynivalenol and fumonisin B3.This proposed method has the advantages of simple operation,low cost,high sensitivity and accuracy,which can be used for the high-throughput screening of multiple mycotoxins in plant-solid beverages.
关键词
QuEChERS/超高效液相色谱串联质谱法/真菌毒素/植物固体饮料/高通量
Key words
QuEChERS/ultra-performance liquid chromatography-tandem mass spectrometry/mycotoxins/plant solid beverages/high-throughput
维普
万方数据