摘要
本文主要研究了西藏来源副干酪乳杆菌对小鼠便秘的润肠通便作用及其对肠道菌群的影响.首先将160只雄性昆明鼠随机分为A、B两大组,每组又随机分为空白组、模型组、5株实验菌株(TI-5、TI-7、TI-9、TI-d、5B-1)组和6108对照菌株组做润肠通便实验,每组10只,其中A组80只用于测定小鼠小肠推进率,B组80只用于排便情况测定.然后挑选润肠通便效果好的菌株TI-5、T1-9、5B-1进行肠道菌群调节的检测,实验小鼠为雄性BALB/c小鼠共24只,分为空白组、T1-5组、T1-9组和5B-1组,每组6只.润肠通便实验结果表明:与模型组相比,T1-9和5B-1组小鼠小肠推进率显著高于模型组(P＜0.01);T1-9组和5B-1组首次红便时间显著缩短(P＜0.01);T1-9组、5B-1组以及对照菌株6108组6h的排便颗粒数显著增加(P＜0.05);T1-9组和6108组的粪便湿重显著提高(P＜0.01、P＜0.05).肠道菌群调节实验的测序结果显示,与空白组相比,三组益生菌组T1-5、T1-9、5B-1有益菌乳杆菌科、理研菌科丰度明显增加,其中5B-1组有益菌普雷沃氏菌科丰度也明显增加.因此菌株T1-5、T1-9、5B-1能够促进肠道蠕动,也具有调节肠道菌群结构发挥缓解小鼠便秘的作用.
Abstract
The effects of five strains of Lactobacillus paracasei from Tibet on constipation and gut microbiota of mice were studied.One hundred and sixty male Kunming mice were randomly divided into two groups:A and B.Each group was randomly divided into a blank subgroup,a model subgroup,five experimental strain(including T1-5,T1-7,T1-9,T1-d,and 5B-1)subgroup and a control strain 6108 subgroup for the laxative function test,with 10 mice in each subgroup.Among them,80 mice in group A were used to measure the small intestine propulsion rate of mice,and 80 mice in group B were used to measure defecation status.Strains T1-5,T1-9,and 5B-1 with good performance in laxative function test were then selected to detect the regulation of intestinal microbiota and total 24 male BALB/c mice were selected for this experiment,and were divided into a blank subgroup,a T1-5 subgroup,T1-9 subgroup,and 5B-1 subgroup,with 6 mice in each subgroup.The experimental results of the laxative function test showed that:Compared with the model group,the small intestine propulsion rate of mice in T1-9 and 5B-1 groups was significantly higher than that of the model group(P＜0.01).The first red stool time was significantly shortened in the T1-9 and 5B-1 groups(P＜0.01).The number of fecal particles in the T1-9 group,5B-1 group,and control strain 6108 group increased significantly at 6 h(P＜0.05).The wet weight of feces in the T1-9 and 6108 groups increased significantly(P＜0.01,P＜0.05).The experimental results of the microbial community sequencing test showed that:compared with the blank group,the abundance of probiotics in the three strain groups T1-5,T1-9,and 5B-1 Lactobacillaceae and Rikenellaceae was significantly increased,including Prevotellaceae in the 5B-1 group.So,the conclusion are as follows:strains T1-5,T1-9 and 5B-1 can promote intestinal peristalsis,regulate the structure of intestinal microbiota,and alleviate constipation in mice.
维普
万方数据