摘要
目的:探讨不同剂量阿里红多糖(Fomes officinalis Ames polysaccharides,FOP)对运动性免疫抑制(Exercise-induced immunosuppression,EIS)大鼠肠道菌群及免疫功能的保护作用.方法:50只7～8周龄SPF级雄性SD大鼠随机分为对照组(NC组)、运动训练组(Ex组)、运动训练+低剂量FOP组(LFOP组,40 mg/(kg·d))、运动训练+中剂量FOP组(MFOP组,60 mg/(kg·d))和运动训练+高剂量FOP组(HFOP组,80 mg/(kg·d)).Ex组和各剂量FOP组采用6周跑台训练构建EIS模型,LFOP、MFOP、HFOP组每次训练后灌胃FOP.干预结束后,酶联免疫吸附试验检测血清免疫球蛋白G(Immunoglobulin G,IgG)、免疫球蛋白M(Immunoglobulin M,IgM)、白介素 6(Interleukin-6,IL-6)、白介素 10(Interleukin-10,IL-10)、干扰素γ(Interferon-y,INF-γ)、肿瘤坏死因子a(Tumornecrosis factor-α,TNF-α)、脂多糖(Lipopolysaccharide,LPS)水平及肠道短链脂肪酸含量;细胞分析仪检测血清CD4+、CD8+的数量;16SrDNA检测大鼠肠道菌群结构变化;Western blot检测结肠组织紧密连接蛋白(Zonula occludens-1,ZO-1)、封闭蛋白(Occludin)、紧密连接蛋白4(Claudin4)蛋白表达.结果:与Ex组比较,LFOP、MFOP、HFOP组大鼠血清IgG、IgM、INF-γ、TNF-α、CD4+和CD8+极显著上升(P＜0.01),血清IL-6、IL-10和LPS水平极显著降低(P＜0.01);肠道短链脂肪酸含量和拟杆菌门、疣微菌门及乳杆菌属、拟杆菌属、毛螺菌属(未分类)、艾克曼菌属相对丰度极显著上升(P＜0.01);结肠组织ZO-1、Occludin、Claudin4蛋白表达极显著上调(P＜0.01).此外,MFOP、HFOP组大鼠脾脏指数、胸腺指数、肠道菌群a多样性较Ex组极显著上升(P＜0.01).结论:FOP可能通过减轻炎症改善肠道菌群结构及活性,提高机体免疫功能,进而抑制EIS的发生发展.
Abstract
Objective:To investigate the protective effects of different doses of Fomes officinalis Ames polysaccharides(FOP)on intestinal flora and immune function in exercise-induced immunosuppression(EIS)rats.Methods:Fifty SPF male SD rats aged 7 to 8 weeks were randomly divided into control group(NC group),exercise training group(Ex group),exercise training+low-dose FOP group(LFOP group,40 mg/(kg·d)),exercise training+medium-dose FOP group(MFOP group,60 mg/(kg·d))and exercise training+high-dose FOP group(HFOP group,80 mg/(kgd)).Ex group and FOP group were trained on the treadmill for 6 weeks to construct EIS model.LFOP,MFOP and HFOP groups were given FOP after each training.After the intervention,serum IgG,IgM,IL-6,IL-10,INF-γ,TNF-α,LPS levels and intestinal short-chain fatty acid content were detected by enzyme-linked immunosorbent assay.The number of serum CD4+and CD8+was detected by cell analyzer.16S rDNA was used to detect the structural changes of intestinal flora.Western blot was used to detect the protein expression of ZO-1,Occludin,Claudin4 in the colon tissue.Results:Compared with Ex group,serum IgG,IgM,INF-γ,TNF-α,CD4+and CD8+of rats in LFOP,MFOP and HFOP groups were significantly increased(P＜0.01),while serum levels of IL-6,IL-10 and LPS were significantly decreased(P＜0.01).Intestinal short-chain fatty acid content and the relative abundance of Bacteroidetes,Verrucobacteria and Lactobacillus,Bacteroidetes,Spirillum(not classified)and Ekmanella were significantly increased(P＜0.01).The expression of ZO-1,Occludin,Claudin4 proteins in colon tissue was significantly up-regulated(P＜0.01).In addition,spleen index,thymus index,intestinal flora alpha diversity in MFOP and HFOP groups were significantly increased compared with Ex group(P＜0.01).Conclusion:FOP may improved the structure and activity of intestinal flora by reducing inflammation,enhanced the body's immune function,and then inhibited the development of EIS.
基金项目
新疆维吾尔自治区喀什地区科技计划(20200024)
新疆维吾尔自治区高校科技计划(XJEDU2021SY040)
维普
万方数据