提取方法对黄精渣水不溶性膳食纤维结构和功能特性的影响

Effects of Extraction Methods on Structural and Functional Properties of Water-insoluble Dietary Fiber from Polygonatum sibiricum Residue

王鑫 ¹课净璇 ²王亚斌 ²孙静 ²刘菡 ¹万鹏 ²马艳莉 ²陈志周¹

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作者信息

1. 河北农业大学食品科技学院,河北保定 071000
2. 南阳理工学院张仲景国医国药学院,河南南阳 473000
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摘要

以黄精渣为原料,探究不同提取方法对黄精渣水不溶性膳食纤维(IDF)理化性质和功能特性的影响.采用复合酶法(CE)、超声辅助复酶法(UCE)和酶碱法(CEA)提取IDF,并考察三种IDF的组成、理化特性、葡萄糖吸附能力、阳离子交换能力和DPPH自由基清除能力等功能特性.结果表明,超声辅助复酶法得率最高(84.68％).三种黄精渣IDF纯度均较高,主要由纤维素、半纤维素和木质素组成,其中UCE-IDF纯度高达82.24％.UCE-IDF 持水力(4.74±0.66)g/g、持油力(3.84±0.29)g/g 和结合水力(3.29±0.09)g/g 最优,总酚含量(7.77±0.07)mg/g最高,阳离子交换能力(0.40±0.003)mmol/g最佳,抗氧化能力最佳.CEA-IDF的膨胀力(5.33±0.11)mL/g 和总黄酮含量(1.88±0.03)mg/g 最高.CE-IDF 的葡萄糖吸附能力(19326.67±41.63)μmol/g最佳.理化表征显示,UCE-IDF结构更加不规则且具有更高的热稳定性.红外光谱显示三种黄精渣IDF均具有纤维素类特征吸收峰.X射线结果显示三种黄精渣IDF呈纤维素I晶型.综上,三种IDF的理化和功能特性之间存在差异,UCE-IDF在持水、持油等多项理化特性中具有明显优势,且热稳定性和抗氧化活性最佳.CEA-IDF的总黄酮含量最高且膨胀力最佳.CE-IDF的葡萄糖吸附能力最佳,本实验结果可为黄精渣IDF在食品加工中的应用提供理论参考.

Abstract

The effects of different extraction methods on the physical and chemical properties and functional characteristics of insoluble dietary fiber(IDF)from Polygonatum sibiricum residue were investigated.The complex enzyme method(CE),ultrasonic-assisted complex enzyme method(UCE)and enzyme-alkali method(CEA)were used to extract IDF.The composition,physicochemical properties,glucose adsorption capacity,cation exchange capacity and DPPH free radical scavenging capacity of three IDFs were analyzed.Results showed that the yield of ultrasound-assisted compound enzyme method was the highest(84.68％).Three IDFs had a higher purity and mainly consisted of cellulose,hemicellulose,and lignin.The purity of UCE-IDF was up to 82.24％.UCE-IDF had the best water holding capacity(4.74±0.66)g/g,oil holding capacity(3.84±0.29)g/g,binding capacity(3.29±0.09)g/g,the highest total phenol content(7.77±0.07)mg/g,the best cation exchange capacity(0.40±0.003)mmol/g,and the antioxidant capacity.CEA-IDF had the highest swelling capacity(5.33±0.11)mL/g and flavonoid content(1.88±0.03)mg/g.CE-IDF had the best glucose adsorption capacity(19326.67±41.63)μmol/g.Physical and chemical characterization indicated that the structure of UCE-IDF was more irregular and had higher thermal stability.The infrared spectra suggested that the three IDFs had the characteristic absorption peaks of cellulose.The X-ray results revealed that the three IDFs were cellulose I crystal.In summary,there were differences in physical and chemical properties and functional characteristics among the three IDFs.UCE-IDF showed significant advantages in several physical and chemical properties,such as water holding,oil holding thermal stability,and antioxidant activity.CEA-IDF was distinguished by its high flavonoid content and the best swelling power.CE-IDF had the best glucose adsorption capacity.The results of this study could provide a theoretical basis for the application of IDF in food processing industry.

关键词

黄精渣/提取方法/水不溶性膳食纤维/理化性质/功能特性

Key words

Polygonatum sibiricum residue/extraction method/insoluble dietary fiber/physical and chemical properties/functional properties

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出版年

2024

食品工业科技

北京一轻研究院

食品工业科技

CSTPCD北大核心

影响因子：0.842

ISSN：1002-0306

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