To investigate the crucial pathways and differentially expressed genes following the action of Ornithogalum saundersiae Saponins(OSW-1)on liver cancer cells(HEP3B),this study utilized transcriptome sequencing technology to examine the gene expression data of HEP3B cells treated with OSW-1.By precisely analyzing the transcripts from the measured sequence information,differentially expressed genes related to cell survival were screened to identify core genes.Furthermore,experiments were conducted to verify whether OSW-1 could treat hepatocellular carcinoma by regulating the core targets.Results showed that the MTT assay revealed a significant reduction in HEP3B cell activity following OSW-1 treatment,which was dependent on the concentration.A total of 1381 genes showed significant differences in expression as a result of transcriptome sequencing,with a majority being involved in the TNF signaling pathway.Additionally,immunofluorescence and Western blot analysis indicated that the levels of p-PI3K,p-AKT,and p-NF-κB proteins were reduced in HEP3B cells after treatment with OSW-1.In conclusion,OSW-1 may suppress inflammation and reduce HEP3B cell activity by modulating inflammatory signaling pathways,suggesting its potential as a drug for liver cancer treatment and providing an experimental foundation for the development of healthy foods aimed at liver protection.
维普
万方数据