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厌氧发酵对云南小粒咖啡香气特征的影响

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本研究以云南小粒咖啡(Coffea arabica L.)为原料,以气相色谱-质谱和电子鼻为主要研究手段,考察厌氧发酵过程中挥发性风味物质的转变,进一步通过多元统计学方法得到香气特征的变化规律.研究发现,发酵过程中呋喃类相对含量从46.88%降至32.5%,酸类相对含量从8.36%增至10.36%、酯类相对含量由6.34%上升至15.38%.通过香气活度值(OAV)筛选出42种芳香活性化合物,其中棕榈酸乙酯、2-糠醛、乙酸是主要芳香化合物.厌氧发酵明显增强了榛子、杏仁、花香的风味特征,发酵3d时具有最明显的酸味,而发酵5 d时花果香气更为突出.苯甲醇与2-吡咯烷酮是发酵样品中特有香气化合物,2-乙酰基吡啶、2-甲基-5-(1-甲基乙烯基)吡啶、反式-2-癸烯醛和丙酸是未发酵样品的特有成分.厌氧发酵时间可用于调控云南小粒咖啡香气特征,特征性香气化合物可以作为监测厌氧发酵进程的标志物.不同发酵时间对咖啡6种香气强度产生了可区分的差异.
Effect of Anaerobic Fermentation on the Aroma Characteristics of Yunnan Arabica Coffee
In this study,Yunnan Arabica coffee(Coffea arabica L.)was used as raw material,with gas chromatography-mass spectrometry(GC-MS)and electronic nose employed as primary techniques to analyze the transformation of volatile flavor compounds during anaerobic fermentation,followed by the application of multivariate statistical methods to elucidate the evolving patterns of aroma characteristics.The results revealed a decrease in the relative content of furan from 46.88%to 32.5%,an increase in acid content from 8.36%to 10.36%,and an increase in ester content from 6.34%to 15.38%.A total of 42 aromatic compounds were screened by aroma activity value(OAV),with ethyl palmitate,2-furfural,and acetic acid being the predominant aromatic compounds.Anaerobic fermentation significantly enhanced the flavor characteristics of hazelnut,almond,and floral notes.Benzyl alcohol and 2-pyrrolidone were specific aroma compounds in fermented samples,while 2-acetylpyridine,2-methyl-5-(1-methylvinyl)pyridine,trans-2-decenal,and propanoic acid are specific aroma compounds in unfermented samples.Anaerobic fermentation time could be manipulated to regulate the aroma characteristics of Yunnan Arabica coffee,where characteristic aromatic compounds could serve as markers for monitoring anaerobic fermentation progress.Different fermentation time had a distinguishable difference on the aroma intensity of 6 kinds of coffee.

Arabica coffeeanaerobic fermentationvolatile compoundgas chromatography-mass spectrometry(GC-MS)electronic nose

鲁翀、于鑫欣、胡发广、付兴飞、赵玉红、董文江

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东北林业大学生命科学学院,黑龙江哈尔滨 150040

中国热带农业科学院香料饮料研究所,海南万宁 571533

海南省特色热带作物适宜性加工与品质控制重点实验室,海南万宁 571533

云南省农业科学院热带亚热带经济作物研究所,云南保山 678600

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小粒咖啡 厌氧发酵 挥发性成分 气相色谱-质谱法(GC-MS) 电子鼻

2025

食品工业科技
北京一轻研究院

食品工业科技

北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2025.46(1)