Isolation and Purification,Structural Characterization,Hypoglycemic Activity of Soluble β-Glucan from Cordyceps chrysanthemi
Soluble β-glucan from mushroom source had good biological activity,in order to develop and utilize β-glucan(CMBG)in Cordyceps chrysanthemi,this study used Cordyceps chrysanthemi substrate as raw material,and was purified by proteolytic enzyme digestion,adsorption of macroporous resin,and chromatography of DEAE ion-exchange columns,and the decolorization rate and β-glucan retention rate were weighted and scored to feel out the establishment of the optimal purification process conditions.The obtained β-glucan was structurally analyzed and its in vitro hypoglycemic activity was investigated.The results showed that the macroporous resin HPD-750 had the best adsorption effect,and the optimal conditions for adsorption were as follows:up-sample flow rate of 5 mL/min,up-sample concentration of 4 mg/mL,up-sample volume of 400 mL,and mass of the resin of 40 g.Two β-glucan fractions,CMBG-1 and CMBG-2,with relative molecular weights of 13.57×104 Da and 14.36× 104 Da,respectively,were isolated using a DEAE-FF cellulose exchange column.Both consisted of three monosaccharides,Gal,Glc,and Man,with molar ratios of 1.28∶5.27∶1.01 and 0.71∶4.43∶0.80,respectively.The Congo red analysis showed that CMBG-1 had a triple-helix structure,while CMBG-2 had no triple-helix structure,and the inhibition rates of both of them on α-amylase and α-glucosidase were positively correlated with the concentrations of the samples,and the IC50 of their inhibition on α-amylase were 2.68 and 4.32 mg/mL,and those on α-glucosidase were 2.37 and 3.01 mg/mL,respectively.The hypoglycemic activity of CMBG-1,which possessed a triple-helix structure,was significantly higher than that of CMBG-2,which did not possess a triple-helix structure,and it had good prospects for the development of functional foods.
Cordyceps militaris β-glucanseparation and purificationstructural analysishypoglycemic activity
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