Analysis of Fungal Diversity and Isolation and Identification of Yeast in High-temperature and Medium-high-temperature Daqu Using Three Generation Sequencing Technology
To elucidate the variations in fungal diversity and cultivable yeast in different types of Daqu,third-generation sequencing technology was employed to analyze the fungal communities in high-temperature and medium-high-temperature Daqu.Additionally,traditional microbial pure culture methods and molecular biology techniques were utilized to investigate the yeast resources within Daqu.Alpha diversity analysis revealed significant differences(P＜0.05)in the richness and diversity of fungal communities between the two types of Daqu.The sequencing data indicated that Thermomyces lanuginosus predominated in high-temperature Daqu,with an average relative content of 73.81％ ,while medium-high-temperature Daqu was primarily composed of Aspergillus proliferans,representing an average relative content of 52.58％ .Beta diversity analysis demonstrated a notable distinction in the fungal community structure between high-temperature and medium-high-temperature Daqu(P＜0.01).Moreover,the LEfSe analysis identified Thermomyces and Aspergillaceae as potential biomarkers for high-temperature and medium-high-temperature Daqu,respectively.Pure culture results revealed 18 yeast strains isolated from 10 Daqu samples,with Saccharomycopsis fibuligera identified as the dominant yeast strain in both high-temperature and medium-high-temperature Daqu.These findings underscore pronounced differences in fungal diversity between high-temperature and medium-high-temperature Daqu.
国家科技期刊平台
NSTL
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