玫瑰花渣多糖提取工艺优化及体外抗氧化活性分析
Optimization of Polysaccharide Extraction Process from Rosa rugosa Residue and Analysis of Its Antioxidant Activity in Vitro
张婷阳 1张伶 2蒋雨秦 3刘洋 4王新惠 4赵欣欣 2苗保河3
作者信息
- 1. 中国农业科学院都市农业研究所/成都农业科技中心,四川成都 610000;成都大学,四川成都 610000
- 2. 中国农业科学院都市农业研究所/成都农业科技中心,四川成都 610000
- 3. 中国农业科学院都市农业研究所/成都农业科技中心,四川成都 610000;中农科(山东)玫瑰生物科技有限责任公司,山东济南 250404
- 4. 成都大学,四川成都 610000
- 折叠
摘要
玫瑰花渣是玫瑰精油提取过程中产生的主要废弃物之一.以玫瑰花渣为原料,采用单因素实验结合Box-Behnken响应面法优化了超声辅助纤维素酶与果胶酶复合提取玫瑰花渣多糖的提取工艺,并对多糖体外抗氧化活性进行了研究.结果表明,在料液比为1∶15,复合酶(纤维素酶∶果胶酶=1∶1)添加量为1.9％,酶解温度47℃,酶解时长84.5 min的条件下,玫瑰花渣多糖得率为4.308％±0.03％.体外抗氧化实验表明,玫瑰花渣多糖总抗氧化能力较强,当多糖溶液浓度为5 mg/mL时,其FRAP值为0.30,对DPPH·、ABTS+·的清除能力较强,清除率分别为98.93％和92.19％,对O2·的清除能力较弱,清除率为24.93％.同时,玫瑰花渣多糖对DPPH·、ABTS+·、O2-·清除能力的IC50值大小分别为0.357、0.608、64.206 mg/mL.该研究为玫瑰花渣的回收再利用提供了理论依据.
Abstract
Rose flower residue,a major by-product of rose essential oil extraction,was used as the raw material in this study.The extraction process of polysaccharides from rose flower residue was optimized using single factor experiments combined with the Box-Behnken response surface method,with ultrasonic assistance and a composite enzyme treatment of cellulase and pectinase.The in vitro antioxidant activity of the polysaccharides was also investigated.Results showed that under the conditions of a material-to-liquid ratio of 1∶15,a composite enzyme addition(cellulase to pectinase at a 1∶1 ratio)of 1.9％ ,an enzymatic hydrolysis temperature of 47 ℃,and a duration of 84.5 min,the yield of polysaccharides from rose flower residue was 4.308％ ±0.03％ .In vitro antioxidant tests showed that the rose flower residue polysaccharides had strong overall antioxidant capacity.At a polysaccharide solution concentration of 5 mg/mL,the FRAP value was 0.30,with strong scavenging abilities against DPPH and ABTS+·,with scavenging rates of 98.93％ and 92.19％ ,respectively.A weaker scavenging ability against O2-·,with a scavenging rate of 24.93％ .Meanwhile,the IC50 value of DPPH·,ABTS+·,O2-·respective clearance capacity was 0.357,0.608,and 64.206 mg/mL.This research provides a theoretical basis for the recycling and reuse of rose flower residue.
关键词
玫瑰花渣/复合酶/多糖/响应面分析/抗氧化活性Key words
Rosa rugose residue/complex enzymes/polysaccharides/response surface analysis/antioxidant activity引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据