Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus
In this study,an ultrasonic synergistic semi-bionic method was explored to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures.By conducting single-factor experiments,Plackett-Burman design,and response surface design,the impact of 12 factors on co-culture intracellular polysaccharide(CC-IPS)extraction content,including extraction material-liquid ratios,extraction time,extraction temperature,and extraction solution pH were investigated.The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+radical scavenging rates.Results showed that,the optimal extraction process parameters for the first,second,and third extractions,respectively,included material-to-liquid ratios of 1∶17,1∶40,and 1∶30(g/mL),extraction times of 31,30,and 30 min,extraction temperatures of 44,40,and 50 ℃,and extraction solution pH levels of 3,8,and 10.Under these optimal conditions,the CC-IPS extraction content was 86.4921±1.1924 mg/g,closed to the predicted value of 85.3981 mg/g.Additionally,the IC50 values of CC-IPS in the DPPH and ABTS+free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL,respectively.This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures.
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