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不同底物的红茶菌发酵液对消化酶活性的影响

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对从红茶菌中分离出的菌株(Gluconacetobacter sp.A4)进行研究,分别以红茶、荷叶、荷叶与山楂、荷叶与决明子为底物进行发酵,以传统红茶菌混合发酵为对照,通过对其产物D-葡萄糖二酸-1,4-内酯含量等指标的测定来评价发酵效果,同时研究了不同底物红茶菌发酵液对消化酶活性的影响。结果表明:在30℃、发酵8 d的情况下,以荷叶山楂A4发酵液发酵最为充分,其次为红茶A4发酵液。红茶A4发酵液的D-葡萄糖二酸-1,4-内酯(DSL)生成量最高。各发酵液对脂肪酶的抑制作用以红茶A4发酵液抑制作用最强,荷叶A4发酵液次之;对α-淀粉酶的抑制作用,以荷叶决明子A4发酵液的抑制率最高,红茶A4发酵液次之。不同红茶菌发酵液对消化酶的活性具有抑制作用,为其减肥机理的研究以及功能饮料的开发奠定基础。
The influence of kombucha fermentation with different substrates on digestive enzyme activity
The objective of this article was to study the indexes and emzyme activity such as lipase and a-amylase of Kombucha tea(KT) fermented by Gluconacetobacter sp.A4 (G. sp. A4) whose substrates were black tea(Group A), lotus leaf(Group H), lotus leaf and hawthorn fruit (Group S), lotus leaf and cassia seed (Group J), as well as traditional KT fermented by mixed tea fungus. The results indicated that Group S had the most sufficient fermention, Group A took the second place. Group A generated the most DSL. KTs showed inhibition to lipase and a-amylase, Group A had the strongest inhibition on lipase and Group H took the second place, Group J had the strongest inhibition on (a-amylase and Group A took the second place. Kombucha fermentation with different substrates displayed inhibition on digesitive enzyme activity, this result provided evidence for the mechanism of anti-obesity and functional beverage development.

KombuchaGluconacetobacter sp. A4amylaselipase

王鸥、李涛、吴薇、籍保平

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中国农业大学食品科学与营养工程学院,北京100083

北京市工商行政管理局密云分局,北京101500

中国农业大学工学院,北京100083

红茶菌 葡萄糖酸醋酸杆菌A4 淀粉酶 脂肪酶

2012

食品科技
北京市粮食科学研究所

食品科技

北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2012.(2)
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