Use 10 mg/L, 20 mg/L and 30 mg/L of natamycin solution to treat the red strawberry fruit and MAP(30%O2+6%CO2)store them at (2±1)℃ The results showed that natamycin treatments effectively controlled the mold population, slowed down the chlorophyll degradation, control the organic acid of the fruits decrease, and extend shelf life of strawberry. It can also improve the fruits quality as well as reserving nutrition. When the strawberry treated by 20 mg/L and 30 mg/L natamycin was storage for 21 d, its rotten rate was only 3.3% and its quality was excellent.